Direct imaging of single-walled carbon nanotubes in cells
Direct imaging of single-walled carbon nanotubes in cells
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DOI：
10.1038/nnano.2007.347
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发表时间：
2007-11-01
影响因子：
38.3
通讯作者：
Welland, Mark
中科院分区：
文献类型：
--
作者：
Porter, Alexandra E.;Gass, Mhairi;Welland, Mark
单壁碳纳米管在生物医学领域的应用前景广阔.然而，关于单壁碳纳米管（1-10）的毒性作用的矛盾数据突出了对替代方法的需要，以研究它们在细胞中的摄取和细胞毒性作用。单壁碳纳米管已显示在许多类型的细胞中具有急性毒性（1-3），但由于难以区分基于碳的纳米管和富含碳的细胞结构，因此先前尚未证实对单壁碳纳米管的细胞摄取的直接观察.在这里，我们使用透射电子显微镜和共聚焦显微镜成像的单壁碳纳米管易位到细胞在染色和未染色的人类细胞。观察到纳米管进入细胞质并定位在细胞核内，以剂量依赖性方式引起细胞死亡。
The development of single- walled carbon nanotubes for various biomedical applications is an area of great promise. However, the contradictory data on the toxic effects of single- walled carbon nanotubes(1-10) highlight the need for alternative ways to study their uptake and cytotoxic effects in cells. Single- walled carbon nanotubes have been shown to be acutely toxic(1-3) in a number of types of cells, but the direct observation of cellular uptake of single- walled carbon nanotubes has not been demonstrated previously due to difficulties in discriminating carbon- based nanotubes from carbon- rich cell structures. Here we use transmission electron microscopy and confocal microscopy to image the translocation of single- walled carbon nanotubes into cells in both stained and unstained human cells. The nanotubes were seen to enter the cytoplasm and localize within the cell nucleus, causing cell mortality in a dose dependent manner.