Antioxidant effect of cysteamine in brain cortex of young rats
Antioxidant effect of cysteamine in brain cortex of young rats
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DOI：
10.1007/s11064-007-9486-7
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发表时间：
2008-05-01
影响因子：
4.4
通讯作者：
Duval Wannmacher, Clovis Milton
中科院分区：
文献类型：
--
作者：
Kessler, Adriana;Biasibetti, Micheli;Duval Wannmacher, Clovis Milton
半胱胺是一种用于治疗半胱氨酸病的半胱氨酸耗竭药物，半胱氨酸病是一种由于溶酶体胱氨酸载体缺乏而引起的代谢紊乱。因此，胱氨酸在包括神经系统在内的许多组织和器官的溶酶体内积累。对半胱氨酸二甲酯负载细胞的研究表明，半胱氨酸可能通过氧化应激诱导细胞凋亡。我们的目的是研究半胱胺和氧化剂半胱氨酸二甲酯联合给药对大鼠大脑皮层氧化应激的几个参数的影响。分别注射1.6mU/g半胱氨酸二甲酯和/或0.26mU/g半胱胺。半胱氨酸二甲酯诱导脂质过氧化、蛋白质羰化，并刺激超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶的活性，可能是通过形成自由基来实现的。半胱胺阻止了这些影响，可能增加了细胞内的硫醇库，并充当了自由基的清除剂。这些结果提示，半胱胺的抗氧化作用可能在治疗胱氨酸病中起重要作用。
Cysteamine is a cystine-depleting drug used in the treatment of cystinosis, a metabolic disorder caused by deficiency of the lysosomal cystine carrier. As a result, cystine accumulates within lysosomes in many tissues and organs, including the nervous system. Studies with cystine dimethyl ester loaded cells suggest that cystine might induce apoptosis through oxidative stress. Our objective was to investigate the effects of co-administration of cysteamine with the oxidant cystine dimethyl ester on several parameters of oxidative stress in the brain cortex of rats. Animals were injected with 1.6 mu mol/g cystine dimethyl ester and/or 0.26 mu mol/g body weight cysteamine. Cystine dimethyl ester induced lipoperoxidation, protein carbonylation, and stimulated superoxide dismutase, glutathione peroxidase and catalase activities, probably through the formation of free radicals. Cysteamine prevented those effects, possibly increasing cellular thiol pool and acting as a scavenger of free radicals. These results suggest that the antioxidant effect of cysteamine may be important in the treatment of cystinosis.