PROCTOLIN AND EXCITATION OF THE CRAYFISH SWIMMERET SYSTEM
PROCTOLIN AND EXCITATION OF THE CRAYFISH SWIMMERET SYSTEM
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DOI：
10.1002/cne.903450411
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发表时间：
1994-07-22
影响因子：
2.5
通讯作者：
MULLONEY, B
中科院分区：
文献类型：
--
作者：
ACEVEDO, LD;HALL, WM;MULLONEY, B
螯虾腹神经索含有5对兴奋性中间神经元的轴突，每对中间神经元都能激活游泳网系统。用神经肽proctolin灌注腹神经索也激活了游泳系统。本实验旨在验证一个或多个这些兴奋性中间神经元使用proctolin作为递质的假设。在一只个体小龙虾中，分别定位和刺激五个兴奋性轴突中的每一个，通过刺激它们中的每一个引起相似的运动活动。通过对自发泳鼠运动模式与刺激这些兴奋性轴突之一E（C）或用proctolin溶液灌注引起的活动进行定量比较，表明在这三种条件下产生的运动模式没有显著差异（P > 0.05）.通过使用新的亲和纯化的proctolin抗血清，我们标记了最后一个胸神经节和第一个腹神经节之间的结缔组织中的轴突，并将标记的轴突的位置与先前描述的兴奋性轴突的位置进行了比较。约0.3%的轴突在这些结缔组织中表现出proctolin样免疫反应，但大量标记的轴突对发生双边兴奋性swimmeret轴突的区域。在连续的塑料切片中追踪这些标记的轴突到腹部神经节的投射。标记的过程是丰富的外侧神经末梢，位点的swimmeret图案生成circuits.From这个证据，我们建议，这些兴奋性swimmeret中间神经元使用proctolin作为一个发射器，但第四不。第五个轴突的证据是模棱两可的。(C)1994 Wiley-Liss，Inc.
The ventral nerve cord of crayfish contains axons of five pairs of excitatory interneurons, each of which can activate the swimmeret system. Perfusion of the ventral nerve cord with the neuropeptide proctolin also activates the swimmeret system. The experiments reported here were conducted to test the hypothesis that one or more of these excitatory interneurons uses proctolin as a transmitter.Each of the five excitatory axons was located and stimulated separately in an individual crayfish, and similar motor activity was elicited by stimulating each of them. Quantitative comparison of spontaneous swimmeret motor patterns with activity caused by stimulating one of these excitatory axons, E(C), or by perfusing with proctolin solutions showed that the motor patterns produced under these three conditions were not significantly different (P > 0.05).By using a new, affinity-purified proctolin antiserum, we labeled axons in the connective tissue between the last thoracic and first abdominal ganglion and compared the positions of labeled axons with the previously described positions of the excitatory axons. About 0.3% of the axons in these connective tissues showed proctolin-like immunoreactivity, but heavily labeled pairs of axons did occur bilaterally in the regions of excitatory swimmeret axons. The projections of these labeled axons into the abdominal ganglia were traced in serial plastic sections. Labeled processes were abundant in the lateral neuropils, the loci of the swimmeret pattern-generating circuitry.From this evidence, we propose that three of these excitatory swimmeret interneurons use proctolin as a transmitter, but that a fourth does not. The evidence for the fifth axon is ambiguous. (C) 1994 Wiley-Liss, Inc.