Direct Relationship Between Metabolic Flexibility Measured During Glucose Clamp and Prolonged Fast in Men
Direct Relationship Between Metabolic Flexibility Measured During Glucose Clamp and Prolonged Fast in Men
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DOI：
10.1002/oby.22783
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发表时间：
2020-05-05
期刊：
影响因子：
6.9
通讯作者：
Galgani, Jose E.
中科院分区：
文献类型：
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作者：
Fernandez-Verdejo, Rodrigo;Castro-Sepulveda, Mauricio;Galgani, Jose E.
目的研究正糖-高胰岛素钳夹期间代谢柔韧性（MetFlex）与长时间禁食的关系。本研究还分析了MetFlex与代谢健康之间的关系。方法18名健康男性（平均[SD]: 22 b[2]岁，BMI: 22 [1] kg/m(2)）进行了两个疗程：(1)正糖-高胰岛素钳夹（2 mIU/kg胰岛素/分钟）和(2)类似于20小时禁食。Clamp MetFlex对应于（Delta）呼吸商（RQ）的变化（Delta RQ =挑战后RQ -挑战前RQ）调整M值和挑战前RQ。延长快速MetFlex与Delta β -羟基丁酸调整后的Delta RQ和预激RQ相对应。结果钳夹期间MetFlex与延长快速期间MetFlex直接相关（r = 0.59, P = 0.014）。利用MetFlex的中位数，本研究将参与者分为高MetFlex和低MetFlex两组。确定了对这两项挑战具有高或低MetFlex的参与者。高MetFlex组受试者血清低密度脂蛋白胆固醇比低MetFlex组受试者低3% （P = 0.021）。结论在钳夹或长时间快速测量MetFlex产生类似的结果，尽管挑战不同底物的氧化。对这些挑战的MetFlex受损可能是脂质代谢异常发展的早期事件。
Objective This study aimed to determine the relationship between metabolic flexibility (MetFlex) measured during a euglycemic-hyperinsulinemic clamp and a prolonged fast. This study also analyzed the association between MetFlex and metabolic health.Methods Eighteen healthy men (mean [SD]: 22 [2] years old; BMI: 22 [1] kg/m(2)) performed two sessions: (1) euglycemic-hyperinsulinemic clamp (2 mIU/kg of insulin per minute) and (2) similar to 20-hour fast. Clamp MetFlex corresponded to the change in (Delta) respiratory quotient (RQ) (Delta RQ = postchallenge RQ - prechallenge RQ) adjusted for M value and prechallenge RQ. Prolonged fast MetFlex corresponded to the Delta RQ adjusted for the Delta beta-hydroxybutyrate and prechallenge RQ.Results MetFlex during the clamp related directly with MetFlex during prolonged fast (r = 0.59, P = 0.014). Using the median of MetFlex for each challenge, this study split participants into high or low MetFlex. Participants with high or low MetFlex to both challenges were identified. Participants with high MetFlex had 3% lower serum low-density lipoprotein cholesterol than participants with low MetFlex (P = 0.021).Conclusions Measuring MetFlex during a clamp or a prolonged fast produces similar results, despite challenging the oxidation of different substrates. An impaired MetFlex in response to these challenges may be an early event in the development of abnormal lipid metabolism.