Synergistic Effects on Incorporation of β-Tricalcium Phosphate and Graphene Oxide Nanoparticles to Silk Fibroin/Soy Protein Isolate Scaffolds for Bone Tissue Engineering
Synergistic Effects on Incorporation of β-Tricalcium Phosphate and Graphene Oxide Nanoparticles to Silk Fibroin/Soy Protein Isolate Scaffolds for Bone Tissue Engineering
复制标题
β-磷酸三钙和氧化石墨烯纳米颗粒掺入丝素蛋白/大豆分离蛋白支架用于骨组织工程的协同效应
DOI：
10.3390/polym12010069
复制
发表时间：
2020-01-01
期刊：
影响因子：
5
通讯作者：
Liu, Yi
中科院分区：
文献类型：
--
作者：
Liu, Fan;Liu, Chen;Liu, Yi
在骨组织工程中，理想的支架材料必须具有良好的物理、化学（或物理化学）和生物学（或生化）特性，以促进成骨。虽然丝素蛋白（SF）和/或大豆蛋白分离物（SPI）支架已被广泛用作自体和异源骨移植物的替代物，但其机械性能差和骨诱导能力不足已成为其体内应用的障碍。本文中，将β-磷酸三钙（β-TCP）和氧化石墨烯（GO）纳米颗粒同时或单独掺入SF/SPI支架中。采用场发射扫描电子显微镜（FESEM）、X射线衍射（XRD）和衰减全反射傅里叶变换红外光谱（ATR-FTIR）对复合支架的理化性能进行了表征。采用骨髓间充质干细胞（BMSCs）评价复合支架的生物相容性和成骨能力。所有的复合支架都具有复杂的多孔结构，具有合适的孔径和孔隙率。通过掺入β-TCP和GO纳米颗粒可以显著增加支架的理化性质。碱性磷酸酶活性（ALP）和成骨相关的基因表达的BMSCs显着增强的β-TCP和GO纳米粒子的存在下。特别是，β-TCP和GO纳米颗粒在促进成骨方面具有协同作用。这些结果表明，β-TCP和GO增强的SF/SPI支架是骨组织再生的有希望的候选者。
In bone tissue engineering, an ideal scaffold is required to have favorable physical, chemical (or physicochemical), and biological (or biochemical) properties to promote osteogenesis. Although silk fibroin (SF) and/or soy protein isolate (SPI) scaffolds have been widely used as an alternative to autologous and heterologous bone grafts, the poor mechanical property and insufficient osteoinductive capability has become an obstacle for their in vivo applications. Herein, beta-tricalcium phosphate (beta-TCP) and graphene oxide (GO) nanoparticles are incorporated into SF/SPI scaffolds simultaneously or individually. Physical and chemical properties of these composite scaffolds are evaluated using field emission scanning electron microscope (FESEM), X-ray diffraction (XRD) and attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR). Biocompatibility and osteogenesis of the composite scaffolds are evaluated using bone marrow mesenchymal stem cells (BMSCs). All the composite scaffolds have a complex porous structure with proper pore sizes and porosities. Physicochemical properties of the scaffolds can be significantly increased through the incorporation of beta-TCP and GO nanoparticles. Alkaline phosphatase activity (ALP) and osteogenesis-related gene expression of the BMSCs are significantly enhanced in the presence of beta-TCP and GO nanoparticles. Especially, beta-TCP and GO nanoparticles have a synergistic effect on promoting osteogenesis. These results suggest that the beta-TCP and GO enhanced SF/SPI scaffolds are promising candidates for bone tissue regeneration.