Glycosphingolipid composition of primary cultured human brain microvascular endothelial cells
Glycosphingolipid composition of primary cultured human brain microvascular endothelial cells
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DOI：
10.1002/jnr.20228
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发表时间：
2004-10
影响因子：
4.2
通讯作者：
T. Kanda;T. Ariga;H. Kubodera;Honglian Jin;K. Owada;T. Kasama;M. Yamawaki;H. Mizusawa
中科院分区：
文献类型：
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作者：
T. Kanda;T. Ariga;H. Kubodera;Honglian Jin;K. Owada;T. Kasama;M. Yamawaki;H. Mizusawa
鞘糖脂(GSL)抗原被认为与包括多发性硬化症在内的自身免疫性神经系统疾病的发病机制有关。为了建立形成血脑屏障(BBB)的内皮细胞的GSL图谱，我们建立了一种高纯度的高纯度人脑微血管内皮细胞(HBMECs)的方法，并将其GSL组成与作为不形成血脑屏障的内皮细胞的代表的人脐静脉内皮细胞(HUVECs)进行了比较。神经节苷脂主要是GM3和唾液酸侧副糖苷(LM1)，中性GSLS主要是乳糖基神经酰胺(LacCer)、三糖神经酰胺(Gb3)和三糖苷(Gb4)。用高效薄层层析法可以检测到痕量的GM1、GD1a、GD1b、GT1b和磺基葡萄糖醛酸侧副糖苷(SGPG)。SGPG只能在非融合状态下检测到，其含量几乎是非融合状态的1/30。反之，融合后GM3和LM1显著增加。HBMECs中Gb3的含量几乎是HUVECs的两倍。HBMECs和HUVECs之间以及融合状态和非融合状态之间GSL含量的这些差异的意义尚不清楚。然而，它可能与血脑屏障的防御机制以及某些以细胞表面GSL为靶点的疾病的中枢神经系统的易感性有关，例如溶血性尿毒症综合征。©2004 Wiley-Liss Inc.
Glycosphingolipid (GSL) antigens have been considered to be involved in the pathogenesis of autoimmune neurologic disorders including multiple sclerosis. To establish the GSL pattern specific for endothelial cells forming blood–brain barrier (BBB), we established a method to yield sufficient quantities of highly purified human brain microvascular endothelial cells (HBMECs) and compared their GSL composition to that of human umbilical cord vein endothelial cells (HUVECs), as the representative of endothelial cells not forming BBB. The major gangliosides were GM3 and sialyl paragloboside (LM1), and the major neutral GSLs were lactosylceramide (LacCer), globotriaosylceramide (Gb3), and globoside (Gb4). Trace amounts of GM1, GD1a, GD1b, GT1b, and sulfoglucuronosyl paragloboside (SGPG) could be detected by the high performance thin layer chromatography‐overlay method. SGPG was detected only at a nonconfluent state in an amount almost 1/30 that of in nonconfluent HUVECs. Conversely, GM3 and LM1 increased significantly after confluency. The amount of Gb3 in HBMECs was almost as twice that in HUVECs. The significance of these differences in GSL content between HBMECs and HUVECs and between confluent and nonconfluent states is obscure. It might be related, however, to the defense mechanism at the BBB and to the susceptibility of the central nervous system in some disorders that target cell surface GSL, such as hemolytic uremic syndrome. © 2004 Wiley‐Liss, Inc.