Proteinase-3 mRNA expressed by glomerular epithelial cells correlates with crescent formation in Wegener's granulomatosis.
Proteinase-3 mRNA expressed by glomerular epithelial cells correlates with crescent formation in Wegener's granulomatosis.
复制标题
肾小球上皮细胞表达的蛋白酶 3 mRNA 与韦格纳肉芽肿病的新月体形成相关。
作者：
A. Schwarting;D. Hagen;M. Odenthal;H. Brockmann;H. Dienes;E. Wandel;H. Rumpelt;K. zum Büschenfelde;P. Galle;W. Mayet
背景
BACKGROUND
Wegener's granulomatosis (WG) is characterized by systemic vasculitis with crescentic glomerulonephritis (CGN) and circulating autoantibodies directed against neutrophil cytoplasmic antigens (ANCA). Proteinase 3 (PR-3), a neutral serine proteinase in neutrophils implicated in the growth control of myeloid cells, has been identified as the target antigen for ANCA in WG. Since the kidneys are frequently involved in WG, we studied the in situ expression of PR-3 by renal parenchymal cells.
METHODS
We assessed the expression of PR-3 in kidney biopsies of 15 patients with WG by immunohistochemistry (IHC) and in situ hybridization (ISH). Normal kidney tissue served as the control.
RESULTS
We detected PR-3 mRNA and PR-3 protein in distal tubular epithelial cells (TECs) and glomerular epithelial cells (GECs) in normal kidney tissue and in CGN. Furthermore, a strong glomerular PR-3mRNA expression restricted to the site of cellular crescents was detected in patients with WG. The analysis of 144 glomeruli with cellular or sclerotic crescents revealed a positive correlation of glomerular PR-3mRNA expression with the percentage of cellular crescents per glomerulus. The capability of human TECs and GECs to synthesize PR-3 was confirmed by Northern blot and ISH on cultured cells.
CONCLUSION
These data provide evidence that nonhematopoetic renal parenchymal cells express PR-3 and that glomerular expression of PR-3 is associated with crescent formation in WG. Our findings suggest that renal parenchymal cells may directly be involved in the pathogenesis of CGN in WG.
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蛋白酶 3 基因的结构、染色体分配和表达。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Sturrock,AB;Franklin,KF;Rao,G;Marshall,BC;Rebentisch,MB;Lemons,RS;Hoidal,JR
通讯作者：
Hoidal,JR
多形核白细胞颗粒蛋白对人肺源性细胞和内皮细胞的直接细胞毒性。
DOI：
10.1164/ajrccm/141.1.179
发表时间：
1990
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Okrent,DG;Lichtenstein,AK;Ganz,T
通讯作者：
Ganz,T
影响因子：
168.9
作者：
FEU, F;GARCIAPAGAN, JC;RODES, J
通讯作者：
RODES, J
影响因子：
15.9
作者：
CAMPANELLI, D;DETMERS, PA;GABAY, JE
通讯作者：
GABAY, JE
影响因子：
5
作者：
Nag, S;Papneja, T;Stewart, DJ
通讯作者：
Stewart, DJ