Improved method for avian influenza virus isolation from environmental water samples
Improved method for avian influenza virus isolation from environmental water samples
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DOI：
10.1111/tbed.14639
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发表时间：
2022-07-06
影响因子：
4.3
通讯作者：
Ozawa, Makoto
中科院分区：
文献类型：
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作者：
Khalil, Ahmed Magdy;Kojima, Isshu;Ozawa, Makoto
在越冬地点对候鸟进行环境水目标监测可能是了解禽流感病毒（AIVs）生态学的最有效方法之一。在这项研究中，我们改进了从环境水样中分离AIV的方法，对我们之前报道的过程进行了轻微的修改。实验证明，改进后的方法的AIV回收率比原方法提高了10-100倍。与之前的冬季相比，这种改进的分离方法使我们能够从2018/2019冬季日本数万只候鸟越冬地点收集的环境水样中分离出大量的AIV分离物。遗传和系统发育分析表明，相同亚型的aiv具有多个遗传星座，在一个冬季在一个越冬地点传播。这些结果表明，我们改进的分离方法有助于加强环境水目标监测，并通过监测正在进行的AIV循环，更好地了解候鸟种群中的AIV生态。
Environmental water-targeted surveillance of migratory aquatic birds at overwintering sites is potentially one of the most effective approaches for understanding the ecology of avian influenza viruses (AIVs). In this study, we improved the method for AIV isolation from environmental water samples by making a minor modification to our previously reported process. We experimentally demonstrated that the AIV recovery efficiency of the modified method was 10-100-fold higher than that of the original method. This improved isolation method allowed us to isolate a considerably larger number of AIV isolates from environmental water samples collected at an overwintering site for tens of thousands of migratory aquatic birds in Japan during the 2018/2019 winter season, compared with those during previous winter seasons. Genetic and phylogenetic analyses revealed that AIVs of the same subtypes with multiple genetic constellations were circulating in a single overwintering site during a single winter season. These findings indicate that our improved isolation method contributes to enhance environmental water-targeted surveillance and to a better understanding of AIV ecology in migratory aquatic bird populations by monitoring ongoing AIV circulation.