A RISE IN CYTOSOLIC CALCIUM IS NOT NECESSARY FOR MATURATION OF XENOPUS-LAEVIS OOCYTES
A RISE IN CYTOSOLIC CALCIUM IS NOT NECESSARY FOR MATURATION OF XENOPUS-LAEVIS OOCYTES
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DOI：
10.1016/0012-1606(87)90136-9
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发表时间：
1987-05-01
影响因子：
2.7
通讯作者：
ROBINSON, KR
中科院分区：
文献类型：
--
作者：
CORK, RJ;CICIRELLI, MF;ROBINSON, KR
利用这种发光蛋白水母发光蛋白测定了非洲爪蟾卵母细胞减数分裂成熟过程中细胞质游离钙水平。[Ca ~（2+）]i静息水平为92.6 ± 0.01。30 nM。没有观察到显着的变化后，孕酮添加，虽然一个大脉冲的[Ca 2 +]i后，观察到激活成熟的卵母细胞。这些研究结果进行了讨论的钙在成熟中的作用，它的结论是，钙不是孕酮的第二信使。这一结论进一步得到以下发现的支持：100 μ M TMB-8（细胞内钙释放的阻断剂）对孕酮诱导的成熟没有影响。
Cytoplasmic free calcium levels during progesterone-induced meiotic maturation in Xenopus laevis oocytes were measured using this photoprotein aequorin. The resting level of [Ca2+]i was 92.6 .+-. 30 nM. No significant changes were observed after progesterone addition, although a large pulse of [Ca2+]i was observed upon activation of matured oocytes. These findings are discussed in terms of the role of calcium in maturation and it is concluded that calcium is not the second messenger for progesterone. This conclusion is further supported by the finding that 100 .mu.M TMB-8, a blocker of intracellular calcium release had no effect on progesterone-induced maturation.