Chromosomal Abnormalities in Human Lymphocytes after Computed Tomography Scan Procedure-猫眼课题宝

DOI：

10.1667/rr14976.1

发表时间：

2018-07

期刊：

Radiation Research

影响因子：

3.4

通讯作者：

中科院分区：

医学3区

文献类型：

作者：

Lin Shi;Kurumi Fujioka;Nami Sakurai-Ozato;W. Fukumoto;K. Satoh;Jiying Sun;A. Awazu;Kimio Tanaka;M. Ishida;T. Ishida;Yukiko Nakano;Y. Kihara;C. N. Hayes;H. Aikata;K. Chayama;Takashi Ito;K. Awai;S. Tashiro

染色体异常的发生率和癌症风险与受到中等至高剂量电离辐射后的辐射剂量密切相关。然而，低于100 mGy的生物效应和健康风险，例如，计算机断层扫描（CT）尚未确定。为了研究CT程序低剂量暴露的生物学效应，我们使用端粒和着丝粒PNA探针进行FISH检测，检查了外周血淋巴细胞（PBL）中的染色体畸变，双着丝粒和环状染色体（dic+ring）。在60例非肿瘤患者中，双着丝粒染色体和环状染色体数目随个体差异而显著增加。采用137 Cs辐射装置对15名健康志愿者进行体外低剂量照射后，采用PNA-FISH技术分析其外周血淋巴细胞，证实了CT检查后双着丝粒染色体和环状染色体增量的个体差异。这些研究结果有力地表明，适当的医疗使用低剂量辐射应考虑辐射敏感性的个体差异。

The incidence of chromosomal abnormalities and cancer risk correlates well with the radiation dose after exposure to moderate- to high-dose ionizing radiation. However, the biological effects and health risks at less than 100 mGy, e.g., from computed tomography (CT) have not been ascertained. To investigate the biological effects of low-dose exposure from a CT procedure, we examined chromosomal aberrations, dicentric and ring chromosomes (dic+ring), in peripheral blood lymphocytes (PBLs), using FISH assays with telomere and centromere PNA probes. In 60 non-cancer patients exposed to CT scans, the numbers of dicentric and ring chromosomes were significantly increased with individual variation. The individual variations in the increment of dicentric and ring chromosomes after CT procedures were confirmed using PNA-FISH analysis of PBLs from 15 healthy volunteers after in vitro low-dose exposure using a 137Cs radiation device. These findings strongly suggest that appropriate medical use of low-dose radiation should consider individual differences in radiation sensitivity.

Chromosomal Abnormalities in Human Lymphocytes after Computed Tomography Scan Procedure

文献摘要

相似文献