Interactions of cells with silk surfaces
Interactions of cells with silk surfaces
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DOI：
10.1039/c2jm31174g
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Scheibel, Thomas
中科院分区：
文献类型：
--
作者：
Leal-Egana, Aldo;Scheibel, Thomas
聚合物在组织工程中经常被用来替代受损的细胞外基质。在过去的几十年里，人们对丝蛋白作为生物聚合物用于制造生物相容性的人工支架进行了广泛的研究。包括低或缺乏免疫反应和缺乏细胞毒性，丝蛋白在组织工程和器官修复中表现出有趣的特性。由于细胞-基质相互作用决定了细胞的行为和移植物的后路整合，因此本文主要关注丝支架的表面特性（润湿性、电荷、弹性和生物降解性）对其上培养的细胞的生物活性（粘附、增殖和/或迁移）的影响。此外，还强调了丝蛋白的来源（天然来源、再生或重组生产）以及支架形态及其处理/后处理如何影响生物医学应用背景下支架表面特性。
Polymers are often employed in tissue engineering to replace damaged extracellular matrix (ECM). During the last few decades silk proteins have been extensively investigated concerning their use as biopolymers for the generation of biocompatible, artificial scaffolds. Including the low or absence of immune-response and lack of cell toxicity, silk proteins present interesting properties useful for tissue engineering and organ repair. Since cell-matrix interactions define the behaviour of cells and posterior graft integration, this review is focused on the influence of surface properties of silk scaffolds (wettability, charge, elasticity and biodegradability) on the biological activity (adhesion, proliferation and/or migration) of cells cultured thereon. Further, it is highlighted how the origin of silk proteins (natural source, regenerated or recombinantly produced), as well as the scaffold morphology and its treatment/post-treatment influence the scaffold surface properties in the context of biomedical applications.