Structural biology of replication initiation factor Mcm10.
Structural biology of replication initiation factor Mcm10.
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DOI：
10.1007/978-94-007-4572-8_11
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发表时间：
2012
影响因子：
--
通讯作者：
Eichman, Brandt F
中科院分区：
文献类型：
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作者：
Du, Wenyue;Stauffer, Melissa E;Eichman, Brandt F
微小染色体维持蛋白10（Mcm 10）是真核生物复制叉正确组装所需的非酶复制因子。Mcm 10与单链和双链DNA、聚合酶α和Mcm 2 -7相互作用，并且对于复制前复合物的激活和在S期开始时将后续蛋白质募集到起始处是重要的。此外，Mcm 10最近被牵连在复制叉进展过程中的解旋酶和聚合酶活动的协调。Mcm 10直接或间接参与这些活动的性质仍然不明。然而，最近从多种生物体中获得的Mcm 10的生物化学和结构表征提供了关于Mcm 10如何利用模块化结构作为复制体支架的见解，这有助于定义在延伸过程中起源DNA解链、pol α募集和酶活性协调中的可能作用。
Minichromosome maintenance protein 10 (Mcm10) is a non-enzymatic replication factor required for proper assembly of the eukaryotic replication fork. Mcm10 interacts with single-stranded and double-stranded DNA, polymerase α, and Mcm2-7, and is important for activation of the pre-replicative complex and recruitment of subsequent proteins to the origin at the onset of S-phase. In addition, Mcm10 has recently been implicated in coordination of helicase and polymerase activities during replication fork progression. The nature of Mcm10's involvement in these activities, whether direct or indirect, remains unknown. However, recent biochemical and structural characterization of Mcm10 from multiple organisms has provided insights into how Mcm10 utilizes a modular architecture to act as a replisome scaffold, which helps to define possible roles in origin DNA melting, pol α recruitment and coordination of enzymatic activities during elongation.