Involvement of Smi1 in cell wall integrity and glucan synthase Bgs4 localization during fission yeast cytokinesis.
Involvement of Smi1 in cell wall integrity and glucan synthase Bgs4 localization during fission yeast cytokinesis.
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DOI：
10.1091/mbc.e21-04-0214
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发表时间：
2022-02-01
影响因子：
3.3
通讯作者：
Wu, Jian-Qiu
中科院分区：
文献类型：
--
作者：
Longo, Larissa V. G.;Goodyear, Evelyn G.;Zhang, Sha;Kudryashova, Elena;Wu, Jian-Qiu
胞质分裂是细胞分裂周期的最后一步。在真菌中，它依赖于肌动球蛋白收缩环的收缩和分裂部位隔膜的构建的协调。葡聚糖糖苷酶合成葡聚糖，葡聚糖是真菌细胞壁和分裂隔膜的主要成分。已知Rho 1和Rho 2 GTP酶调节葡聚糖酶Bgs 1、Bgs 4和Ag 1，Sbg 1和F-BAR蛋白Cdc 15在Bgs 1稳定性和向质膜的递送中起作用。在这里，我们的特点是Smi 1，一种内在的无序蛋白质，与BGS 4相互作用，并调节其在裂变酵母中的运输和定位。Smi 1对隔膜的完整性很重要，它的缺失会导致胞质分裂过程中严重的细胞溶解。Smi 1定位于分泌囊泡，并与Bgs 4一起向分裂位点移动。葡聚糖酶Bgs 1和Bgs 4和葡聚糖酶Agn 1和Bgl 2的浓度在Smi 1突变体的分裂位点处降低，但Smi 1似乎对Bgs 4更特异。将Smi 1错误定位于线粒体会使Bgs 4而非Bgs 1发生错误定位。总之，我们的数据揭示了一种新的调节葡聚糖糖苷酶和葡聚糖酶，Smi 1，这是更重要的BGS 4的运输，稳定性和定位在胞质分裂。
Cytokinesis is the final step of the cell-division cycle. In fungi, it relies on the coordination of constriction of an actomyosin contractile ring and construction of the septum at the division site. Glucan synthases synthesize glucans, which are the major components in fungal cell walls and division septa. It is known that Rho1 and Rho2 GTPases regulate glucan synthases Bgs1, Bgs4, and Ags1, and that Sbg1 and the F-BAR protein Cdc15 play roles in Bgs1 stability and delivery to the plasma membrane. Here we characterize Smi1, an intrinsically disordered protein that interacts with Bgs4 and regulates its trafficking and localization in fission yeast. Smi1 is important for septum integrity, and its absence causes severe lysis during cytokinesis. Smi1 localizes to secretory vesicles and moves together with Bgs4 toward the division site. The concentrations of the glucan synthases Bgs1 and Bgs4 and the glucanases Agn1 and Bgl2 decrease at the division site in the smi1 mutant, but Smi1 seems to be more specific to Bgs4. Mistargeting of Smi1 to mitochondria mislocalizes Bgs4 but not Bgs1. Together, our data reveal a novel regulator of glucan synthases and glucanases, Smi1, which is more important for Bgs4 trafficking, stability, and localization during cytokinesis.
影响因子：
3.7
作者：
de León N;Sharifmoghadam MR;Hoya M;Curto MÁ;Doncel C;Valdivieso MH
通讯作者：
Valdivieso MH