Enhanced human bone marrow mesenchymal stem cell functions in novel 3D cartilage scaffolds with hydrogen treated multi-walled carbon nanotubes
Enhanced human bone marrow mesenchymal stem cell functions in novel 3D cartilage scaffolds with hydrogen treated multi-walled carbon nanotubes
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DOI：
10.1088/0957-4484/24/36/365102
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发表时间：
2013-09-13
期刊：
影响因子：
3.5
通讯作者：
Zhang, Lijie Grace
中科院分区：
文献类型：
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作者：
Holmes, Benjamin;Castro, Nathan J.;Zhang, Lijie Grace
软骨组织是一种纳米结构的组织，由于其固有的再生能力极差和复杂的分层结构而难以再生。目前的治疗方法是高度侵入性的，可能有许多并发症。因此，这项工作的目标是使用纳米材料和纳米/微加工方法来创建新的生物启发组织工程软骨支架，以促进人骨髓间充质干细胞（MSC）软骨形成。为此，我们利用静电纺丝技术设计和制造了一系列基于氢（H-2）处理的多壁碳纳米管（MWCNTs）和生物相容性聚l -乳酸（PLLA）聚合物的新型三维仿生纳米结构支架。具体而言，本研究制备了一系列可控制纤维尺寸的电纺丝纤维PLLA支架。体外MSC研究表明，干细胞倾向于在纤维直径较小的支架上附着。更重要的是，MWCNT嵌入支架的机械强度和压缩杨氏模量与天然软骨相匹配。此外，我们的MSC分化结果表明，H2处理的碳纳米管和聚l -赖氨酸涂层的掺入可以诱导比对照组更多的MSC软骨分化。经过两周的培养，H2处理的MWCNTs和聚l -赖氨酸的PLLA支架可以达到最高的糖胺聚糖合成，这使其有望进一步探索软骨再生。
Cartilage tissue is a nanostructured tissue which is notoriously hard to regenerate due to its extremely poor inherent regenerative capacity and complex stratified architecture. Current treatment methods are highly invasive and may have many complications. Thus, the goal of this work is to use nanomaterials and nano/microfabrication methods to create novel biologically inspired tissue engineered cartilage scaffolds to facilitate human bone marrow mesenchymal stem cell (MSC) chondrogenesis. To this end we utilized electrospinning to design and fabricate a series of novel 3D biomimetic nanostructured scaffolds based on hydrogen (H-2) treated multi-walled carbon nanotubes (MWCNTs) and biocompatible poly(L-lactic acid) (PLLA) polymers. Specifically, a series of electrospun fibrous PLLA scaffolds with controlled fiber dimension were fabricated in this study. In vitro MSC studies showed that stem cells prefer to attach in the scaffolds with smaller fiber diameter. More importantly, the MWCNT embedded scaffolds showed a drastic increase in mechanical strength and a compressive Young's modulus matching to natural cartilage. Furthermore, our MSC differentiation results demonstrated that incorporation of the H2 treated carbon nanotubes and poly-L-lysine coating can induce more chondrogenic differentiations of MSCs than controls. After two weeks of culture, PLLA scaffolds with H2 treated MWCNTs and poly-L-lysine can achieve the highest glycosaminoglycan synthesis, making them promising for further exploration for cartilage regeneration.