THE ROLE OF MACROPHAGES IN PARTICLE TRANSLOCATION FROM LUNGS TO LYMPH-NODES
THE ROLE OF MACROPHAGES IN PARTICLE TRANSLOCATION FROM LUNGS TO LYMPH-NODES
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DOI：
10.1126/science.4071052
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
BICE, DE
中科院分区：
文献类型：
--
作者：
HARMSEN, AG;MUGGENBURG, BA;BICE, DE
红色荧光微球和绿色荧光微球被注入狗肺叶不同但相邻的区域。7天后，引流两个灌注区的气管-支气管部淋巴结内含有许多全红或全绿微球的巨噬细胞，但很少两者兼有。这表明微粒没有被动转运，肺巨噬细胞吞噬了肺内的微球，并将其带到气管、支气管处的淋巴结。此外，两组肺泡巨噬细胞(PAM‘’S)，一组在体内吞噬了红色微球，另一组在吞噬了绿色微球，从狗的肺灌洗，混合成一个群体，并注入回相同狗的未暴露的肺叶。与第一个实验一样，引流灌注区的气管、支气管淋巴结含有大量的巨噬细胞，它们要么全是红色，要么全是绿色微球。这表明，注入PAM的S已迁移到气管、支气管壁的淋巴结。因此，肺巨噬细胞，包括PAM‘’S，可能通过确定颗粒移位在诱导肺免疫和预防疾病中发挥关键作用。
Red fluorescent and green fluorescent microspheres were instilled into separate but adjacent areas of dog lung lobes. After 7 days, the tracheobronchial lymph nodes that drained both of the instilled areas contained many macrophages with all red or all green microspheres but rarely both. This indicates that the particles did not translocate passively and that lung macrophages phagocytized the microspheres in the lung and carried them to the tracheobronchial lymph nodes. In addition, two populations of pumonary alveolar macrophages (PAM''s), one that had phagocytized red microspheres in vivo and one that had phagocytized green microspheres, were lavaged from the lungs of dogs, mixed into one population, and instilled back into a previously unexposed lung lobe of the same dogs. As in the first experiment, the tracheobronchial lymph nodes that drained the instilled area contained numerous macrophages with either all red or all green microspheres. This suggested that the instilled PAM''s had migrated to the tracheobronchial lymph nodes. Thus, lung macrophages, including PAM''s, probably play a critical role in the induction of lung immunity and in protection from disease by determining particle translocation.