ACCUMULATION OF SULFURIC ACID IN DICTYOTALES (PHAEOPHYCEAE): TAXONOMIC DISTRIBUTION AND ION CHROMATOGRAPHY OF CELL EXTRACTS
ACCUMULATION OF SULFURIC ACID IN DICTYOTALES (PHAEOPHYCEAE): TAXONOMIC DISTRIBUTION AND ION CHROMATOGRAPHY OF CELL EXTRACTS
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硫酸在盘藻目（褐藻纲）中的积累：细胞提取物的分类分布和离子色谱分析
DOI：
10.1046/j.1529-8817.1999.3540732.x
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发表时间：
1999
影响因子：
2.9
通讯作者：
H. Kawai
中科院分区：
文献类型：
--
作者：
H. Sasaki;H. Kataoka;M. Kamiya;H. Kawai
Dictyotales目的4种(Dictyopteris latiuscula (Okamura) Okamura， D. prolifera （Okamura) Okamura， D. repens (Okamura) Børgesen和Spatoglossum crassum J. Tanaka）与Desmarestiales （Phaeophyceae）的某些物种一样具有高酸性。通过在蒸馏水中测量细胞提取物的pH值，估计细胞内的pH值为0.5至0.9，这可能是液泡的pH值。然而，这些属的其他种（D. divaricata (Okamura) Okamura， D. undulata Holmes和S. pacificum Yendo）没有表现出高酸度。细胞提取物的离子层析表明，这些物种的细胞中含有高浓度的SO，可达海水中的10倍，但Cl−相对较低。检测到的阳离子（Na+， NH, K+, Mg2+, Ca2+）的总和明显低于阴离子（Cl−,Br−，NO， SO）的总和，这种差异被认为代表质子（H+），导致极低的细胞液pH。从pH数据计算出的细胞质子浓度与从阳离子和阴离子之和和阴离子的差异计算出的细胞质子浓度大致一致。虽然某些其他非酸性的二齿藻物种也含有高浓度的SO，但这些物种含有高浓度的Mg2+，阳离子和阴离子的总和是平衡的。
Four species in the order Dictyotales (Dictyopteris latiuscula (Okamura) Okamura, D. prolifera (Okamura) Okamura, D. repens (Okamura) Børgesen, and Spatoglossum crassum J. Tanaka) were found to be highly acidic as in some species of the order Desmarestiales (Phaeophyceae). The pH within their cells, presumably that of the vacuole, was estimated to be 0.5 to 0.9 by pH measurements of their cell extracts in distilled water. However, other species of these genera (D. divaricata (Okamura) Okamura, D. undulata Holmes, and S. pacificum Yendo) did not show high acidity. Ion chromatography of the cell extracts showed that those species contained high concentrations of SO within their cells, up to 10 times that in seawater but relatively low Cl−. The sum of cations examined (Na+, NH, K+, Mg2+, Ca2+) was significantly lower than that of anions (Cl−, Br−, NO, SO), and the difference is presumed to represent protons (H+), causing the extremely low cell sap pH. Estimated cellular proton concentrations calculated from the pH data roughly agreed with those calculated from differences between the sum of cations and anions and that of anions. Although certain other, nonacidic, dictyotalean species also contained high concentrations of SO, these species contained high concentrations of Mg2+, and the sums of cations and anions were balanced.