Shoot Regeneration Capacity Destined at an Early Stage of Protoplast Culture in Cabbage
Shoot Regeneration Capacity Destined at an Early Stage of Protoplast Culture in Cabbage
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卷心菜原生质体培养早期阶段的芽再生能力
DOI：
10.1270/jsbbs1951.37.22
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发表时间：
1987
期刊：
影响因子：
2.4
通讯作者：
K. Takayanagi
中科院分区：
文献类型：
--
作者：
T. Nishio;H. Yamagishi;K. Takayanagi
研究了培养基中激素平衡的影响，以获得甘蓝、甘蓝原生质体培养中可重复的方法。在原生质体培养的每个步骤中测试了培养基中的不同激素组合，即。 e.液体培养基中的细胞分裂、琼脂培养基上的愈伤组织形成以及琼脂培养基上的愈伤组织的芽再生。芽再生的频率不仅受到再生培养基（最后一步）中植物激素的影响，而且还受到愈伤组织形成步骤培养基中植物激素的影响。在含有0.5mg/l 2、4-D和5mg/l激动素的培养基中发育的愈伤组织表现出最高的芽再生能力，而在含有2mg/l 2、4-D和1mg/l激动素的培养基中发育的愈伤组织再生出很少的芽。与2、4-D和有时分化的根相比，用NAA作为生长素培养的愈伤组织在移植到再生培养基上之前表现出相对较低的再生能力。首先出现根的愈伤组织导致枝条再生非常差。根据这些结果，认为源自原生质体的愈伤组织的芽再生能力是在培养的早期阶段确定的。
Effect of hormonal balance in the culture media was investigated for getting reproducible method in protoplast culture of cabbage, Brassica oleracea L. Different hormonal combinations in the media were tested at each step of protoplast culture, i. e. cell division in liquid media, callus formation on agar media, and shoot regeneration from callus on agar media. Frequency of shoot regeneration was affected by phytohormones not only in the regeneration media (a final step), but also in the media for callus formation step. Callus developed in the medium with 0.5 mg/l 2, 4-D and 5 mg/l kinetin showed the highest shoot regeneration capacity, while those developed in the medium with 2 mg/l 2, 4-D and 1mg/l kinetin regenerated few shoots. Callus cultured with NAA as an auxin showed relatively low regeneration capacity compared that with 2, 4-D, and sometimes differentiated roots before transplanting onto the regeneration medium. Calluses with roots appearing first resulted in very poor shoot regeneration. From these results, shoot regeneration capacity of calluses derived from protoplasts was considered to be determined at an early stage of culture.