Spatiotemporal dynamics of epileptiform propagations: Imaging of human brain slices
Spatiotemporal dynamics of epileptiform propagations: Imaging of human brain slices
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DOI：
10.1016/j.neuroimage.2011.05.046
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发表时间：
2011-09-01
期刊：
影响因子：
5.7
通讯作者：
Kakita, Akiyoshi
中科院分区：
文献类型：
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作者：
Kitaura, Hiroki;Hiraishi, Tetsuya;Kakita, Akiyoshi
癫痫发作活动通常起源于大脑皮层的局部区域，并扩散到大脑的大片区域。这些传播的异常放电的性质可以解释癫痫患者的临床表型，尽管它们的传播方式和潜在机制还不清楚。在本研究中，我们进行了黄素蛋白荧光成像的皮质脑切片手术切除的患者部分癫痫引起的各种症状的病变。诱发癫痫的组织中的神经活动暂时水平地扩散到皮层，但那些来自没有癫痫病史的脑肿瘤患者的对照组织中的神经活动只表现出局部反应。典型的癫痫样传播包括早期和晚期。当刺激强度逐渐改变时，早期表现为全或无反应，而晚期表现为反应逐渐增加。此外，这两个相位通过不同的皮层传播，表明它们来自不同的神经回路。形态学研究显示存在肥大的神经元和树突棘的损失，这可能参与了黄素蛋白荧光成像观察到的异常活动。这些发现表明，同步活动的早期阶段可能发挥了关键作用，在人类皮层癫痫的传播异常放电。(C)2011 Elsevier Inc. All rights reserved.
Seizure activities often originate from a localized region of the cerebral cortex and spread across large areas of the brain. The properties of these spreading abnormal discharges may account for clinical phenotypes in epilepsy patients, although the manner of their propagation and the underlying mechanisms are not well understood. In the present study we performed flavoprotein fluorescence imaging of cortical brain slices surgically resected from patients with partial epilepsy caused by various symptomatic lesions. Elicited neural activities in the epileptogenic tissue spread horizontally over the cortex momentarily, but those in control tissue taken from patients with brain tumors who had no history of epilepsy demonstrated only localized responses. Characteristically, the epileptiform propagation comprised early and late phases. When the stimulus intensity was changed gradually, the early phase showed an all-or-none behavior, whereas the late phase showed a gradual increase in the response. Moreover, the two phases were propagated through different cortical layers, suggesting that they are derived from distinct neural circuits. Morphological investigation revealed the presence of hypertrophic neurons and loss of dendritic spines, which might participate in the aberrant activities observed by flavoprotein fluorescence imaging. These findings indicate that synchronized activities of the early phase may play a key role in spreading abnormal discharges in human cortical epilepsies. (C) 2011 Elsevier Inc. All rights reserved.