Identification of a novel acetylated form of branched-chain polyamine from a hyperthermophilic archaeon Thermococcus kodakarensis
Identification of a novel acetylated form of branched-chain polyamine from a hyperthermophilic archaeon Thermococcus kodakarensis
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DOI：
10.1080/09168451.2017.1345616
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发表时间：
2017-01-01
影响因子：
1.6
通讯作者：
Fujiwara, Shinsuke
中科院分区：
文献类型：
--
作者：
Hidese, Ryota;Im, Ki-Hwan;Fujiwara, Shinsuke
长/支链多胺是在嗜热菌中发现的独特的聚阳离子。嗜热古生菌热球菌体含有亚精胺和支链多胺N-4-双(氨丙基)亚精胺作为主要的多胺。与支链多胺相关的代谢途径仍不清楚。在这里，我们利用气相色谱和液-质联用分析鉴定了一个新的乙酰化多胺，N-4-二(氨丙基)-N-1-乙酰亚精胺，该多胺在其他微生物中没有发现。支链多胺及其乙酰化形式的量随着温度的升高而增加，表明支链多胺在较高温度下对生长是重要的。细胞中N-4-双(氨丙基)亚精胺的量与产生的季乙酰多胺的量有关。在高温胁迫条件下，乙酰化与非乙酰化的比例在稳定期高于对数生长期。
Long/branched-chain polyamines are unique polycations found in thermophiles. The hyperthermophilic archaeon Thermococcus kodakarensis contains spermidine and a branched-chain polyamine, N-4-bis(aminopropyl)spermidine, as major polyamines. The metabolic pathways associated with branched-chain polyamines remain unknown. Here, we used gas chromatography and liquid chromatography-tandem mass spectrometry analyses to identify a new acetylated polyamine, N-4-bis(aminopropyl)-N-1-acetylspermidine, from T. kodakarensis; this polyamine was not found in other micro-organisms. The amounts of branched-chain polyamine and its acetylated form increased with temperature, indicating that branched-chain polyamines are important for growth at higher temperatures. The amount of quaternary acetylated polyamine produced was associated with the amount of N-4-bis(aminopropyl)spermidine in the cell. The ratio of acetylated to non-acetylated forms was higher in the stationary phase than in the logarithmic growth phase under high-temperature stress condition.