EFFICACY OF 7 RETROGRADE TRACERS, COMPARED IN MULTIPLE-LABELING STUDIES OF FELINE MOTONEURONS
EFFICACY OF 7 RETROGRADE TRACERS, COMPARED IN MULTIPLE-LABELING STUDIES OF FELINE MOTONEURONS
复制标题
DOI：
10.1016/0165-0270(94)90142-2
复制
发表时间：
1994-07-01
影响因子：
3
通讯作者：
THOMSON, DB
中科院分区：
文献类型：
--
作者：
RICHMOND, FJR;GLADDY, R;THOMSON, DB
在切断神经暴露或肌肉注射到连续划分的颈部肌肉，颈二腹肌后，评价了7种逆行示踪剂的标记效果。测试的示踪剂包括固蓝（FB）、荧光金（FC）、与荧光素缀合的葡聚糖（FD）、与罗丹明缀合的葡聚糖（Fluoruby（FR），3000和10，000 MW）、荧光乳胶微球、与胶体金偶联的辣根过氧化物酶和1，1 '-双十八烷基-3，3，3'，3 '-四甲基吲哚羰花青高氯酸盐（DiI）。在2只动物中，还采用辣根过氧化物酶，并处理脊髓的过氧化物酶活性，以评价其对荧光示踪剂标记细胞外观的影响。在本实验条件下，在运动神经元中可观察到FB、FG、FD和FR四种示踪剂。FB和FG标记的运动神经元切割神经暴露后的数量相当，但不同的肌肉注射后的数量。注射后FG广泛扩散，不仅在相应的同侧节段的运动神经元中发现，而且在邻近的同侧和对侧节段中也发现。肌内注射FB通常标记细胞比切断神经暴露，但假标记肌内注射后的证据也可以发现。FD或FR标记运动神经元切断神经暴露后，但不肌内注射。结合葡聚糖标记的细胞数量比FB或FG更多，但标记的细胞具有相似的分布模式。其余示踪剂是无效的逆行标记在我们的研究中，这些失败的可能原因进行了讨论。
The labelling efficacies of 7 retrograde tracers were evaluated following cut nerve exposure or intramuscular injection into the serially compartmentalized neck muscle, biventer cervicis. Tested tracers included Fast Blue (FB), Fluorogold (FC), dextran conjugated to fluorescein (FD), dextran conjugated to rhodamine (Fluororuby (FR), 3000 and 10,000 MW), fluorescent latex microspheres, horseradish peroxidase coupled to colloidal gold, and 1,1'-dioctadecyl-3,3,3',3'-tetramethyl indocarbocyanine perchlorate (DiI). In 2 animals, horseradish peroxidase was also employed and spinal cords were processed for peroxidase activity to evaluate its effect on the appearance of cells labelled with fluorescent tracers. Four tracers, FB, FG, FD and FR, could be observed in motoneurones under the conditions of our study. FB and FG labelled comparable numbers of motoneurones following cut nerve exposure, but dissimilar numbers following intramuscular injection. FG diffused extensively following injection and was found in motoneurones not only in the appropriate ipsilateral segment but also adjacent ipsilateral and contralateral segments. Intramuscular injections of FB usually labelled fewer cells than cut nerve exposure, but evidence for spurious labelling following intramuscular injection could also be found. FD or FR labelled motoneurones following cut nerve exposure but not following intramuscular injection. The conjugated dextrans labelled more variable numbers of cells than FB or FG, but the labelled cells had similar patterns of distribution. The remaining tracers were ineffective as retrograde markers in our study, and the possible reasons for these failures are discussed.