Mass spectrometry imaging of therapeutics from animal models to three-dimensional cell cultures.
Mass spectrometry imaging of therapeutics from animal models to three-dimensional cell cultures.
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DOI：
10.1021/acs.analchem.5b00419
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发表时间：
2015-10-06
影响因子：
7.4
通讯作者：
Hummon AB
中科院分区：
文献类型：
--
作者：
Liu X;Hummon AB
质谱仪成像(MSI)是一种用于研究分子在复杂表面的空间分布的强大的无标记技术，已被广泛应用于制药科学，以了解不同药物及其代谢物在从细胞模型到组织的各种生物样品中的分布。在这里，我们回顾了微孔板技术在动物模型药物研究中的应用现状，并讨论了微孔板技术在三维细胞培养中用于准确、高效和高通量分析以评估治疗效果的新进展。
Mass spectrometry imaging (MSI) is a powerful label-free technique for the investigation of the spatial distribution of molecules at complex surfaces and has been widely used in the pharmaceutical sciences to understand the distribution of different drugs and their metabolites in various biological samples, ranging from cell-based models to tissues. Here, we review the current applications of MSI for drug studies in animal models, followed by a discussion of the novel advances of MSI in three-dimensional (3D) cell cultures for accurate, efficient and high-throughput analyses to evaluate therapeutics.