Production of cloned pigs from in vitro systems
Production of cloned pigs from in vitro systems
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DOI：
10.1038/80242
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发表时间：
2000-10-01
影响因子：
46.9
通讯作者：
Bishop, M
中科院分区：
文献类型：
--
作者：
Betthauser, J;Forsberg, E;Bishop, M
在这里，我们描述了一个程序克隆猪的体外培养系统的使用。从两个窝出生四个健康的雄性仔猪培养体细胞的核移植和随后的胚胎移植。另外5例妊娠的启动证明了该程序的可重复性。其重要特征包括核移植前胎儿细胞的体外培养，以及卵母细胞的体外成熟和激活以及体外胚胎培养。本文所述的细胞培养和核移植技术应允许使用遗传修饰程序从克隆猪中产生免疫原性降低的组织和器官，用于异种移植。
Here we describe a procedure for cloning pigs by the use of in vitro culture systems. Four healthy male piglets from two litters were born following nuclear transfer of cultured somatic cells and subsequent embryo transfer. The initiation of five additional pregnancies demonstrates the reproducibility of this procedure. Its important features include extended in vitro culture of fetal cells preceding nuclear transfer, as well as in vitro maturation and activation of oocytes and in vitro embryo culture. The cell culture and nuclear transfer techniques described here should allow the use of genetic modification procedures to produce tissues and organs from cloned pigs with reduced immunogenicity for use in xenotransplantation.