Ionization energies of multiply protonated polypeptides obtained by tandem ionization in Fourier transform mass spectrometers
Ionization energies of multiply protonated polypeptides obtained by tandem ionization in Fourier transform mass spectrometers
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DOI：
10.1002/jms.376
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发表时间：
2002-11-01
影响因子：
2.3
通讯作者：
Zubarev, RA
中科院分区：
文献类型：
--
作者：
Budnik, BA;Tsybin, YO;Zubarev, RA
在4.7和9.4 T傅立叶变换质谱仪的Penning池中通过电子电离测定[M+zH]（z+）（z+）电喷雾产生的多肽的电离能（IE）。对于z=1+和物质P，所发现的IE值为11.0+/-0.4 eV，与先前对于用基质辅助激光解吸/电离产生的离子所获得的IE值一致。对于较高的z，发现以下值：2+[Arg-8]-加压素为11.7+/-0.3 eV，2+ P物质为11.1+/-0.6 eV，2+肾素底物为12.2+/-0.7 eV，胰岛素B链3+为13.3+/-0.4eV，蜂毒肽4+为14.6+/-0.6eV，5+为15.1+/-0.4eV。发现在1.0- 3.5kDa质量范围内的多肽的90%的现有IE数据由经验方程IE（z）=9.8+1.1z描述，其不确定度小于或等于0.5eV。1.1 eV/电荷的平均IE增加归因于库仑排斥。推导出的中性多肽分子的电离能为9.8+/-0.3eV，与文献预期一致。版权所有（C）2002约翰威利父子有限公司
Ionization energies (IE) of [M+zH](z+) (z+) electrospray-produced polypeptides were determined by electron ionization in a Penning cell of 4.7 and 9.4 T Fourier transform mass spectrometers. For z=1+ and substance P, the found IE value of 11.0+/-0.4 eV is in agreement with that obtained earlier for ions generated with matrix-assisted laser desorption/ionization. For higher z, the following values were found: 11.7+/-0.3 eV for 2+ of [Arg-8]-vasopressin, 11.1+/-0.6 eV for 2+ of substance P, 12.2+/-0.7 eV for 2+ of renin substrate, 13.3+/-0.4 eV for 3+ of B-chain of insulin and 14.6+/-0.6 eV for 4+ and 15.1+/-0.4 eV for 5+ of melittin. It was found that 90% of existing IE data on polypeptides in the 1.0-3.5 kDa mass range are described with less than or equal to0.5 eV uncertainty by the empirical equation IE(z)=9.8+1.1z. The average IE increase of 1.1 eV/charge is attributed to Coulombic repulsion. The deduced ionization energy of a neutral polypeptide molecule, 9.8+/-0.3 eV, is consistent with literature expectations. Copyright (C) 2002 John Wiley Sons, Ltd.