A discrete population of mononuclear phagocytes detected by monoclonal antibody
A discrete population of mononuclear phagocytes detected by monoclonal antibody
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单克隆抗体检测到的离散单核吞噬细胞群
DOI：
10.1128/iai.29.2.520-525.1980
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发表时间：
1980
影响因子：
3.1
通讯作者：
S. Russell
中科院分区：
文献类型：
--
作者：
P. A. Leblanc;H. Katz;S. Russell
用鼠骨髓单克隆抗体间接免疫荧光法检测小鼠单核吞噬细胞的抗原决定簇。该抗原表达在粘附的、后期培养的骨髓巨噬细胞和通过注射巯基乙酸盐肉汤引起的慢性炎症性腹腔巨噬细胞上。未检测到抗体与常驻腹膜或肺泡巨噬细胞、血液单核细胞或新鲜分离的骨髓细胞的结合。不到10%的急性炎症单核吞噬细胞表达抗原。该抗体未与淋巴细胞、粒细胞、红细胞、成纤维细胞或几种鼠肿瘤系的细胞可检测地结合。提示与肥大细胞结合的结果不明确。该抗原具有种属特异性，但不具有株系特异性。结论是，成熟，至少，需要抗原的表达。结果表明，额外的影响也参与。
Rat monoclonal antibody raised against cultured mouse bone marrow was used to detect an antigenic determinant on a discrete population of mouse mononuclear phagocytes by indirect immunofluorescence. The antigen was expressed on adherent, late-cultured bone marrow macrophages and chronic inflammatory peritoneal macrophages elicited by the injection of thioglycolate broth. Binding of the antibody to resident peritoneal or alveolar macrophages, blood monocytes, or freshly explanted bone marrow cells was not detected. Less than 10% of acute inflammatory mononuclear phagocytes expressed the antigen. The antibody did not bind detectably to lymphocytes, granulocytes, erythrocytes, fibroblasts, or the cells of several murine tumor lines. Results suggesting binding to mast cells were equivocal. The antigen was species, but not strain, specific. It was concluded that maturation, at least, was required for expression of the antigen. Results suggested that additional influences were also involved.