Direct coupling of transcription and mRNA processing through the thermogenic coactivator PGC-1
Direct coupling of transcription and mRNA processing through the thermogenic coactivator PGC-1
复制标题
DOI：
10.1016/s1097-2765(00)00031-9
复制
发表时间：
2000-08-01
期刊：
影响因子：
16
通讯作者：
Spiegelman, BM
中科院分区：
文献类型：
--
作者：
Monsalve, M;Wu, ZD;Spiegelman, BM
转录和mRNA加工是体内的耦合事件，但协调这些过程的机制在很大程度上是未知的。PGC-1是一种转录辅激活因子，在调节适应性产热中起主要作用。PGC-1还具有某些剪接因子特征的基序。我们在这里证明，在丝氨酸和丝氨酸丰富的结构域和RNA识别基序的PGC-1干扰PGC-1诱导靶基因的mRNA的能力的突变。这些突变也破坏了PGC-1与RNA加工因子共定位和缔合的能力。PGC-1可以改变mRNA的加工，但只有当它加载到基因的启动子上时。这些数据表明通过PGC-1协调调节RNA转录和加工。
Transcription and mRNA processing are coupled events in vivo, but the mechanisms that coordinate these processes are largely unknown. PGC-1 is a transcriptional coactivator that plays a major role in the regulation of adaptive thermogenesis. PGC-1 also has certain motifs characteristic of splicing factors. We demonstrate here that mutations in the serine- and arginine-rich domain and RNA recognition motif of PGC-1 interfere with the ability of PGC-1 to induce mRNAs of target genes. These mutations also disrupt the ability of PGC-1 to co-localize and associate with RNA processing factors. PGC-1 can alter the processing of an mRNA, but only when it is loaded onto the promoter of the gene. These data demonstrate the coordinated regulation of RNA transcription and processing through PGC-1.