The use of transformed T cell lines for clonal expansion of human B cells from peripheral blood, spleen, and tumor-infiltrating lymphocytes.
The use of transformed T cell lines for clonal expansion of human B cells from peripheral blood, spleen, and tumor-infiltrating lymphocytes.
复制标题
使用转化的 T 细胞系从外周血、脾脏和肿瘤浸润淋巴细胞中克隆扩增人类 B 细胞。
作者：
Barbuto,JA;Verastegui,EL;Hersh,EM
人转化T细胞系能够诱导多克隆B细胞活化和免疫球蛋白（IG）从外周血单个核细胞、脾细胞和肿瘤浸润淋巴细胞（TIL）分泌。来自所测试的细胞系之一MOT的细胞不需要任何外源刺激来诱导最大应答，并且在类似条件下诱导比外周血T细胞或其他T细胞系更高水平的应答。MOT诱导的B细胞活化和IG分泌需要细胞接触和MOT培养上清液中存在的因子。MOT细胞在三种测试的肿瘤（黑素瘤、卵巢癌和结肠癌）中诱导来自TIL的B细胞应答，并通过来自HIV+患者的脾细胞诱导HIV特异性免疫球蛋白分泌。
Human transformed T cell lines were able to induce polyclonal B cell activation and immunoglobulin (Ig) secretion from peripheral blood mononuclear cells, spleen cells and tumor-infiltrating lymphocytes(TIL). Cells from one of the lines tested, MOT, did not require any exogenous stimuli to induce maximal responses and under similar conditions induced higher levels of response than peripheral blood T cells or other T cell lines. MOT-induced B cell activation and Ig secretion required cell contact and factors present in the MOT culture supernatant. MOT cells induced B cell responses from TIL in the three tumors tested (melanoma, ovarian and colon cancer) and HIV-specific immunoglobulin secretion by spleen cells from an HIV+ patient.