TARGETING THE E1 REPLICATION PROTEIN TO THE PAPILLOMAVIRUS ORIGIN OF REPLICATION BY COMPLEX-FORMATION WITH THE E2 TRANSACTIVATOR
TARGETING THE E1 REPLICATION PROTEIN TO THE PAPILLOMAVIRUS ORIGIN OF REPLICATION BY COMPLEX-FORMATION WITH THE E2 TRANSACTIVATOR
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DOI：
10.1126/science.2176744
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发表时间：
1990-12-21
期刊：
影响因子：
56.9
通讯作者：
BOTCHAN, MR
中科院分区：
文献类型：
--
作者：
MOHR, IJ;CLARK, R;BOTCHAN, MR
转录因子刺激真核生物DNA复制的机制尚不清楚。牛乳头瘤病毒DNA合成需要病毒E1基因和E2基因编码的转录激活蛋白的产物。实验数据表明，68-kD的E1蛋白与48-kD的E2转录因子形成复合物。该复合物特异性地结合到病毒复制起点，其含有E2的多个结合位点。阻遏蛋白编码的E2开放阅读框未能与E1复合，这表明E2的162个氨基酸区域参与反式激活包含与E1相互作用的关键决定因素。复制蛋白和转录因子之间的物理关联表明转录激活蛋白可以在将复制起始蛋白靶向至其各自的复制起点中起作用。
The mechanism by which transcription factors stimulate DNA replication in eukaryotes is unknown. Bovine papillomavirus DNA synthesis requires the products of the viral E1 gene and the transcriptional activator protein encoded by the E2 gene. Experimental data showed that the 68-kilodalton (kD) E1 protein formed a complex with the 48-kD E2 transcription factor. This complex bound specifically to the viral origin of replication, which contains multiple binding sites for E2. Repressor proteins encoded by the E2 open reading frame failed to complex with E1 suggesting that the 162-amino acid region of E2 that participates in transactivation contained critical determinants for interaction with E1. The physical association between a replication protein and a transcription factor suggests that transcriptional activator proteins may function in targeting replication initiator proteins to their respective origins of replication.