Binding Gibbs energy of ionic liquids to calf thymus DNA: a fluorescence spectroscopy study.
Binding Gibbs energy of ionic liquids to calf thymus DNA: a fluorescence spectroscopy study.
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DOI：
10.1039/c0cp01815e
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发表时间：
2011-02
期刊：
影响因子：
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通讯作者：
Huiyong Wang;Jianji Wang;Shibiao Zhang
中科院分区：
文献类型：
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作者：
Huiyong Wang;Jianji Wang;Shibiao Zhang
用芘探针测定了[C(n)mim]Br （n = 4,6,8,10,12）和[C(4)mim][BF(4)]与小牛胸腺DNA-D1501的结合常数和结合吉布斯能。结果表明，结合吉布斯能随烷基链长度的增加而线性减小。在此基础上，本文报道了DNA与- ch(2)-相互作用以及DNA与阳离子头基相互作用的吉布斯能。结果表明，DNA与分子链阳离子头基之间的静电相互作用是分子链与DNA结合的主要原因，而DNA与分子链烷基链之间的非静电相互作用的贡献则随着分子链烷基链长度的增加而增加。本文所获得的信息可用于设计新型的基于il的DNA提取/分离系统。
The binding constants and binding Gibbs energies of [C(n)mim]Br (n = 4, 6, 8, 10, 12) and [C(4)mim][BF(4)] to calf thymus DNA-D1501 have been determined by the fluorescence measurements of the pyrene probe. It was found that values of the binding Gibbs energy decrease linearly with the increase of alkyl chain length of the ILs. Based on this observation, Gibbs energies for both the interaction between DNA and -CH(2)- of the ILs and the interaction between DNA and the cationic head group of the ILs have been reported. The result suggests that electrostatic interaction between DNA and the cationic head group of the ILs is predominant for the binding of the ILs with DNA although contribution from the non-electrostatic interaction between DNA and the alkyl chain of the ILs increases with increasing alkyl chain length of the ILs. The information obtained here may have application in the design of novel ILs-based DNA extraction/separation systems.