Cloning, characterization, and evolution of the NBS-LRR-encoding resistance gene analogue family in polyploid cotton (Gossypium hirsutum L.)
Cloning, characterization, and evolution of the NBS-LRR-encoding resistance gene analogue family in polyploid cotton (Gossypium hirsutum L.)
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DOI：
10.1094/mpmi.2004.17.11.1234
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发表时间：
2004-11-01
影响因子：
3.5
通讯作者：
Zhang, HB
中科院分区：
文献类型：
--
作者：
He, LM;Du, CG;Zhang, HB
富含亮氨酸的核苷酸结合位点重复序列(NBS-LRR)编码基因家族是目前植物抗病基因研究的热点，目前已克隆的植物抗病基因中约有75%来自该基因家族。我们用聚合酶链式反应的方法从多倍体棉花中克隆了NBS-LRR编码基因。从NBS-LRR基因序列文库中选择150个克隆进行测序，共鉴定出61个抗性基因同源序列。序列分析表明，RGA在棉花基因组中含量丰富且高度分化，根据其核苷酸序列的相似性可分为10个不同的亚家族。不同亚家族的成员数差异很大，基因指数分析表明，每个亚家族处于RGA家族进化的不同阶段。对一组RGA的遗传作图表明，RGA位于数量有限的棉花染色体上，单个亚科的RGA有聚集的趋势，其中两个RGA座位与棉花白叶枯病抗性基因共定位。RGA在棉花A和D两个亚组间的分布是不均匀的，A亚组中RGA的含量高于D亚组。这些数据为多倍体植物中编码NBS-LRR的RGA家族的组织和进化提供了新的见解。
The nucleotide-binding site-leucine-rich repeat (NBS-LRR)-encoding gene family has attracted much research interest because approximately 75% of the plant disease resistance genes that have been cloned to date are from this gene family. We cloned the NBS-LRR-encoding genes from polyploid cotton by a polymerase chain reaction-based approach. A sample of 150 clones was selected from the NBS-LRR gene sequence library and was sequenced, and 61 resistance gene analogs (RGA) were identified. Sequence analysis revealed that RGA are abundant and highly diverged in the cotton genome and could be categorized into 10 distinct subfamilies based on the similarities of their nucleotide sequences. The numbers of members vary many fold among different subfamilies, and gene index analysis showed that each of the subfamilies is at a different stage of RGA family evolution. Genetic mapping of a selection of RGA indicates that the RGA reside on a limited number of the cotton chromosomes, with those from a single subfamily tending to cluster and two of the RGA loci being colocalized with the cotton bacterial blight resistance genes. The distribution of RGA between the two subgenomes A and D of cotton is uneven, with RGA being more abundant in the A subgenome than in the D subgenome. The data provide new insights into the organization and evolution of the NBS-LRR-encoding RGA family in polyploid plants.