Chromatin interaction mechanism of transcriptional control in vivo
Chromatin interaction mechanism of transcriptional control in vivo
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DOI：
10.1093/emboj/17.20.6020
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发表时间：
1998-10-15
期刊：
影响因子：
11.4
通讯作者：
Fraser, P
中科院分区：
文献类型：
--
作者：
Gribnau, J;de Boer, E;Fraser, P
我们已经使用了动力学分析，以区分可能的机制激活转录的不同基因在人类β珠蛋白基因座。基于在单细胞水平的原位研究，我们以前提出了一个动态的机制，单基因交替相互作用的基因座控制区（LCR）激活转录。然而，这些稳态实验不允许直接测量机制的动力学，并且具有来自顺式的两个β样基因的原位初级转录物信号的位点的存在使得该位点中的多个基因可以同时启动转录的可能性是开放的。与RNA FISH结合的涉及去除转录延伸的阻断的动力学测定显示，多个β基因的顺式初级转录信号代表单个基因的交替转录期之间的过渡，支持动态相互作用机制。
We have used a kinetic analysis to distinguish possible mechanisms of activation of transcription of the different genes in the human beta globin locus. Based on in situ studies at the single-cell level we have previously suggested a dynamic mechanism of single genes alternately interacting with the locus control region (LCR) to activate transcription. However, those steady-state experiments did not allow a direct measurement of the dynamics of the mechanism and the presence of loci with in situ primary transcript signals from two beta-like genes in cis has left open the possibility that multiple genes in the locus could initiate transcription simultaneously. Kinetic assays involving removal of a block to transcription elongation in conjunction with RNA FISH show that multiple beta gene primary transcript signals in cis represent a transition between alternating transcriptional periods of single genes, supporting a dynamic interaction mechanism.