Cancer-associated fibroblasts in papillary thyroid carcinoma
Cancer-associated fibroblasts in papillary thyroid carcinoma
复制标题
DOI：
10.1007/s10238-023-00998-2
复制
发表时间：
2023-01
影响因子：
4.6
通讯作者：
Li Zhu;Xuemei Zhang;Shuhan Zhang;Qi-cheng Zhang;Limin Cao;Yujie Zhang;Dan Wang;Xiaohui Liang;Weiming Wu;Shuang Wu;Ruoyu Jiang;Yue Liu;Xue Zhao;Gui-ming Zhou;Ke Xu;Z. Meng
中科院分区：
文献类型：
--
作者：
Li Zhu;Xuemei Zhang;Shuhan Zhang;Qi-cheng Zhang;Limin Cao;Yujie Zhang;Dan Wang;Xiaohui Liang;Weiming Wu;Shuang Wu;Ruoyu Jiang;Yue Liu;Xue Zhao;Gui-ming Zhou;Ke Xu;Z. Meng
甲状腺乳头状癌（PTC）预后相对较好，但也有部分浸润性PTC临床病理特征较差，预后较差。癌相关成纤维细胞（CAFs）在肿瘤的侵袭和转移中起重要作用。本研究旨在通过免疫组织化学方法研究CAFs标志蛋白在PTC中的表达及其与临床病理特征的关系。回顾性分析125例PTC患者的临床资料，并对其标本进行免疫组化染色。对4种CAFs标志蛋白FAP成纤维细胞活化蛋白（FAP）、α-平滑肌肌动蛋白（α-SMA）、波形蛋白（Vimentin）和血小板源性生长因子受体-α（PDGFR-α）进行染色和评分。然后进行统计学分析。FAP和α-SMA免疫反应性评分与肿瘤大小、BRAF突变、甲状腺外浸润、病理分型、淋巴结转移和ATA危险分层相关。二元Logistic回归分析和受试者工作特征曲线显示，FAP和α-SMA免疫反应性评分高是甲状腺外浸润、BRAF突变、多灶性和淋巴结转移（尤其是N1 b）的危险因素，具有良好的敏感性和准确性。发现FAP的性能优于α-SMA。CAFs的高表达是甲状腺癌临床病理特征恶化的危险因素。FAP是PTC较好的CAFs标志物。
Papillary thyroid carcinoma (PTC) has a relatively good prognosis, yet there are some invasive PTC cases with worse clinicopathological features and poor outcome. Cancer-associated fibroblasts (CAFs) play an important role in cancer invasion and metastasis. This study aimed to investigate the expression of marker proteins of CAFs in PTC and their correlations with clinicopathological features through immunohistochemistry. The medical records of 125 PTC patients were reviewed in this study, whose specimens were retrieved for immunohistochemistry. Four CAFs marker proteins, FAP fibroblast activated protein (FAP), α-smooth muscle actin (α-SMA), Vimentin and platelet-derived growth factor receptor-α(PDGFR-α), were stained and scored. Then, statistical analyses were performed. The immunoreactivity scores of FAP and α-SMA correlated with tumor size, BRAF mutation, extrathyroidal, invasion, pathological subtype, lymph node metastasis and ATA risk stratification. Moreover, binary logistic regression analysis and receiver operating characteristic curves showed that high FAP and α-SMA immunoreactivity scores were risk factors for extrathyroidal invasion, BRAF mutation, multi-focality and lymph node metastasis (especially N1b) with good sensitivity and accuracy in prediction. A better performance was found in FAP than α-SMA. Strong expressions of CAFs were risk factors for worse thyroid cancer clinicopathological features. FAP was the better CAFs marker for PTC.