Effects of Red Wine and Vodka on Collateral-Dependent Perfusion and Cardiovascular Function in Hypercholesterolemic Swine
Effects of Red Wine and Vodka on Collateral-Dependent Perfusion and Cardiovascular Function in Hypercholesterolemic Swine
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DOI：
10.1161/circulationaha.111.082172
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发表时间：
2012-09-11
期刊：
影响因子：
37.8
通讯作者：
Sellke, Frank W.
中科院分区：
文献类型：
--
作者：
Chu, Louis M.;Lassaletta, Antonio D.;Sellke, Frank W.
研究背景：适量饮酒，尤其是红酒，已被证明可降低心脏病风险。我们使用了高胆固醇血症的猪慢性缺血模型，以研究2酒精饮料对heart.Methods和Results-Yorkshire猪喂高胆固醇饮食的影响进行了左回旋ameroid收缩放置诱导慢性缺血在8周龄。一组（HCC，n=9）继续单独饮食，第二组（HCW，n=8）补充红葡萄酒（黑比诺，12.5%酒精，每日375 mL），第三组（HCV，n=9）补充伏特加（40%酒精，每日112 mL）。7周后，测量心功能，并收集缺血心肌用于分析灌注、心肌纤维化、血管功能、蛋白表达、氧化应激和毛细血管密度。血小板聚集功能测定。微球注射测量的缺血区域的灌注显着增加HCW和HCV相比，HCC在休息，但只有在HCW组心室起搏。HCW组对5 '-二磷酸腺苷的微血管舒张反应和缺血区域的局部收缩性均得到改善，但HCW和HCV组的心肌纤维化均减少。HCW和HCV中促血管生成蛋白磷酸化内皮一氧化氮合酶和血管内皮生长因子的表达增加，而磷酸化哺乳动物雷帕霉素靶蛋白仅在HCV组中增加。Sirt-1和下游抗氧化剂磷酸化FoxO 1的表达仅在HCW组中增加。蛋白质氧化应激在HCW组单独降低，而毛细血管密度仅在HCV组增加。有没有显着差异，血小板功能groups.Conclusion-moderate消费的红葡萄酒和伏特加酒可能会降低心血管疾病的风险，通过不同的机制，改善依赖于侧支循环的灌注。红葡萄酒可以提供与其抗氧化特性相关的心脏保护。（循环。2012;126[suppl 1]：S65-S72.）
Background-Moderate consumption of alcohol, particularly red wine, has been shown to decrease cardiac risk. We used a hypercholesterolemic swine model of chronic ischemia to examine the effects of 2 alcoholic beverages on the heart.Methods and Results-Yorkshire swine fed a high-cholesterol diet underwent left circumflex ameroid constrictor placement to induce chronic ischemia at 8 weeks of age. One group (HCC, n=9) continued on the diet alone, the second (HCW, n=8) was supplemented with red wine (pinot noir, 12.5% alcohol, 375 mL daily), and the third (HCV, n=9) was supplemented with vodka (40% alcohol, 112 mL daily). After 7 weeks, cardiac function was measured, and ischemic myocardium was harvested for analysis of perfusion, myocardial fibrosis, vessel function, protein expression, oxidative stress, and capillary density. Platelet function was measured by aggregometry. Perfusion to the ischemic territory as measured by microsphere injection was significantly increased in both HCW and HCV compared with HCC at rest, but in only the HCW group under ventricular pacing. Microvessel relaxation response to adenosine 5'-diphosphate was improved in the HCW group alone as was regional contractility in the ischemic territory, although myocardial fibrosis was decreased in both HCW and HCV. Expression of proangiogenic proteins phospho-endothelial nitric oxide synthase and vascular endothelial growth factor was increased in both HCW and HCV, whereas phospho-mammalian target of rapamycin was increased only in the HCV group. Expression of Sirt-1 and downstream antioxidant phospho-FoxO1 was increased only in the HCW group. Protein oxidative stress was decreased in the HCW group alone, whereas capillary density was increased only in the HCV group. There was no significant difference in platelet function between groups.Conclusion-Moderate consumption of red wine and vodka may reduce cardiovascular risk by improving collateral-dependent perfusion through different mechanisms. Red wine may offer increased cardioprotection related to its antioxidant properties. (Circulation. 2012;126[suppl 1]:S65-S72.)