Characterization of 34 polymorphic EST-SSR markers in Tegillarca granosa and their transferability in Anadara craticulata
Characterization of 34 polymorphic EST-SSR markers in Tegillarca granosa and their transferability in Anadara craticulata
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DOI：
10.3724/sp.j.1231.2013.38307
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发表时间：
2013
影响因子：
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通讯作者：
Yinghui Dong;Guo-xing Wu;H. Yao;Pingjiang Bian;Demin Zhao;Zhihua Lin
中科院分区：
文献类型：
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作者：
Yinghui Dong;Guo-xing Wu;H. Yao;Pingjiang Bian;Demin Zhao;Zhihua Lin
EST-SSR是分离微卫星位点的重要方法之一，具有成本低、在近缘物种间可转移性高、与基因组功能区相关性高等优点。34个SSR标记在浙江奉化群体的30个个体中的等位基因数在2 ~ 7个之间，平均每个位点有3.59个等位基因，观察杂合度在0.000 ~ 0.600之间，期望杂合度在0.078 ~ 0.771之间，多态信息含量在0.106 ~ 0.718之间，从已标记的基因中筛选出25个EST-SSR标记，其中8个标记在红斑鳢中表现多态性，据我们所知，这是国内首次报道的血蛤SSR标记的通用性研究，这些新的多态性EST-SSR标记在血蛤的比较定位、基因标签和QTL定位等方面具有重要的应用价值。
EST-SSR is one of important methods to isolate microsatellite loci,which is low cost,high transferability among phylogenetically related species and a probability of being associated with functional regions of the genome.34 polymorphic EST-SSR markers were isolated and characterized in the blood clam,Tegillarca granosa,from the ESTs generated by 454 sequencing.The number of alleles for the 34 SSR markers in 30 individuals of Zhejiang Fenghua population varied from 2 to 7,with an average of 3.59 alleles per locus.The observed heterozygosity ranged from 0.000 to 0.600,while the expected heterozygosity varied from 0.078 to 0.771.Polymorphic information content ranged from 0.106 to 0.718,and 13 SSR loci deviated from Hardy-Weinberg equilibrium.25 EST-SSR identified from annotated genes were expected to be useful for mapping these genes in linkage maps.Interspecific transferability of the 34 markers revealed that 8 were polymorphic in Anadara craticulata,resulting in a transferability rate of 23.53%.To our knowledge,this is the first report on the survey of SSR transferability in blood clams.These novel polymorphic EST-SSR markers should be particularly useful for comparative mapping,gene tagging and QTL mapping among blood clams.