Lamina propria macrophages in the human gastrointestinal mucosa: Their distribution, immunohistological phenotype, and function
Lamina propria macrophages in the human gastrointestinal mucosa: Their distribution, immunohistological phenotype, and function
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DOI：
10.1177/44.7.8675993
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发表时间：
1996-07-01
影响因子：
3.2
通讯作者：
Takahashi, T
中科院分区：
文献类型：
--
作者：
Nagashima, R;Maeda, K;Takahashi, T
在这项研究中，我们系统地研究了巨噬细胞的细胞分布，免疫组织化学表型和粘膜处置功能在人类胃肠道粘膜的层层中（lamina propria巨噬细胞； LPMS），在所有组织中，这些LPM在所有组织中，大多数这些LPM都积累这些细胞表达了覆盖粘膜层层的顶端普通巨噬细胞标记的正常水平，例如CD68，LN5，溶菌酶，铁蛋白和α（1）-Anti-Chymotrypsin。此外，它们表达了高水平的25F9（巨噬细胞的某些亚群的标记），MHC II类分子和CD74（MHC II类相关的不变链），有趣的是，LPM具有某些上皮细胞相关的抗原抗原，例如细胞毒素， ，细胞质中的癌脑抗原（CEA）和BER-EP4。超结构上，这些抗原与LPM摄入的细胞碎片相关，这些抗原通过原位终端标记识别为凋亡片段，此外，通过双重阳性颗粒可通过双重染色，可通过双染色，可通过双染色用于上皮细胞相关的抗原和现场抗原抗原和现场抗原。 - 标签，这些观察结果表明，LPM的主要功能之一是处置凋亡上皮细胞和LPM可能与粘膜上皮更新的调节有关。
In this study we systematically investigated the cellular distribution, immunohistochemical phenotype, and mucosal disposal function of macrophages in the lamina propria of the human gastrointestinal mucosa (lamina propria macrophages; LPMs), In all tissues examined, most of these LPMs accumulated beneath the epithelial layer that covered the apex of the lamina propria of the mucosa, These cells expressed normal levels of common macrophage markers such as CD68, LN5, lysozyme, ferritin, and alpha(1)-anti-chymotrypsin. In addition, they expressed high levels of 25F9 (a marker for a certain subpopulation of macrophages), MHC Class II molecules, and CD74 (MHC Class II-associated invariant chain), Interestingly, LPMs possessed some epithelial cell-associated antigens such as cytokeratin, carcinoembryonic antigen (CEA), and Ber-Ep4 in their cytoplasm. Ultrastructurally, these antigens were associated with cellular debris ingested by LPMs, which were recognized as apoptotic fragments by in situ end-labeling, Furthermore, double positive-labeled granules were seen in LPMs by double staining for epithelial cell-associated antigens and in situ end-labeling, These observations suggest that one of the major functions of LPMs is the disposal of apoptotic epithelial cells and that LPMs may be involved in the regulation of mucosal epithelial renewal.