Association of two different repetitive DNA elements near immunoglobulin light chain genes.
Association of two different repetitive DNA elements near immunoglobulin light chain genes.
复制标题
免疫球蛋白轻链基因附近两个不同重复 DNA 元件的关联。
DOI：
10.1093/nar/11.6.1803
复制
发表时间：
1983
影响因子：
14.9
通讯作者：
Storb,U
中科院分区：
文献类型：
--
作者：
Wilson,R;Storb,U
小鼠免疫球蛋白CK区基因的约6 kb 3'的重复DNA元件的序列研究表明，R元件位于那里（Gebhard等人（1982）J. Mol. B1ol.157.，453-471）与500个碱基对长的元件相邻，该元件与Fanning（Nuc.（1982），10，5003-5013）。BAM 5元件和R元件本身都没有被直接重复包围，但复合元件（BAMS + R）1被15个碱基对的直接重复包围（有一个错配）。直接重复序列由围绕重复DNA元件的靶位点序列组成，被认为是在该位点插入元件期间产生的。因此，似乎BAM 5和R元素作为链接图元进行了交互和插入。在小鼠γ链基因座中存在其它BAM 5/R复合物，表明BAM 5-R协同作用并非罕见事件。
Sequence studies of repetitive DNA elements approximately 6 kb 3’ of the mouse Immunoglobulin CKregion gene show that the R element located there (Gebhard et al. (1982) J. Mol. B1ol.157., 453-471) is adjacent to a 500 base pair long element which shows 80% homology to the BAM5 element sequenced by Fanning (Nuc. Acids Res. (1982),l0, 5003-5013). Neither the BAM5 element nor the R element itself is surrounded by a direct repeat, but the composite element (BAMS + R) 1s surrounded by a 15 base pair direct repeat (with one mismatch). Direct repeats, consisting of target site sequences that surround a repetitive DNA element, are thought to arise during the insertion of the element at that site. It therefore appears that the BAM5 and R elements Interacted and Inserted as a linked entity. The existence of other BAM5/R composites throughout the mouse γ chain locus Indicates that BAM5–R cooperation 1s not a rare event.