MSH5, A NOVEL MUTS HOMOLOG, FACILITATES MEIOTIC RECIPROCAL RECOMBINATION BETWEEN HOMOLOGS IN SACCHAROMYCES-CEREVISIAE BUT NOT MISMATCH REPAIR
MSH5, A NOVEL MUTS HOMOLOG, FACILITATES MEIOTIC RECIPROCAL RECOMBINATION BETWEEN HOMOLOGS IN SACCHAROMYCES-CEREVISIAE BUT NOT MISMATCH REPAIR
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DOI：
10.1101/gad.9.14.1728
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发表时间：
1995-07-15
影响因子：
10.5
通讯作者：
HALSEY, C
中科院分区：
文献类型：
--
作者：
HOLLINGSWORTH, NM;PONTE, L;HALSEY, C
利用设计用于鉴定减数分裂期间同源染色体之间重组特异性缺陷的酵母突变体的筛选，我们获得了减数分裂特异性基因HOP1， RED1和MEK1的新等位基因。此外，筛选还发现了一个名为MSH5 （MutS同源物5）的新基因。尽管Msh5p与MutS蛋白家族具有很强的同源性，但它并不参与DNA错配修复。缺乏MSH5基因的二倍体表现出孢子活力降低，减数分裂I染色体不分离水平增加，同源物之间而不是同源物内部相互交换水平降低。基因转换不会减少。Msh5突变体在表型上与减数分裂特异性基因MSH4的突变体相似（Ross-Macdonald and Roeder 1994）。利用msh4 - msh5二倍体进行的双突变体分析表明，这两个基因在相同的上位群中，因此可能在相似的过程中起作用，即在减数分裂期间促进同源间交叉。
Using a screen designed to identify yeast mutants specifically defective in recombination between homologous chromosomes during meiosis, we have obtained new alleles of the meiosis specific genes, HOP1, RED1, and MEK1. In addition, the screen identified a novel gene designated MSH5 (MutS homolog 5). Although Msh5p exhibits strong homology to the MutS family of proteins, it is not involved in DNA mismatch repair. Diploids lacking the MSH5 gene display decreased levels of spore viability, increased levels of meiosis I chromosome nondisjunction, and decreased levels of reciprocal exchange between, but not within, homologs. Gene conversion is not reduced. Msh5 mutants are phenotypically similar to mutants in the meiosis-specific gene MSH4 (Ross-Macdonald and Roeder 1994). Double mutant analysis using msh4 msh5 diploids demonstrates that the two genes are in the same epistasis group and therefore are likely to function in a similar process-namely, the facilitation of interhomolog crossovers during meiosis.