Association between tortilla consumption and human urinary fumonisin B1 levels in a Mexican population
Association between tortilla consumption and human urinary fumonisin B1 levels in a Mexican population
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DOI：
10.1158/1055-9965.epi-07-2534
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发表时间：
2008-03-01
影响因子：
3.8
通讯作者：
Wild, Christopher P.
中科院分区：
文献类型：
--
作者：
Gong, Yun Yun;Torres-Sanchez, Luisa;Wild, Christopher P.
伏马菌素是由镰刀菌产生的真菌毒素，通常污染世界各地的玉米和玉米产品。伏马菌素是啮齿动物致癌物，与人类食道癌有关。然而，缺乏有效的暴露生物标志物阻碍了人类暴露评估和疾病风险评估。建立了一种灵敏的液相色谱-质谱法，用于测定Oasis MAX药筒提取后的尿伏马菌素B1 (FB1)，并将其应用于墨西哥莫雷洛斯州一组妇女的尿液样本。将尿FB1与食用玉米饼的饮食信息进行比较。用氘标记FB1内标法测定，加标样品中FB1的回收率平均为94%。根据低、中、高玉米基玉米饼的食用量选择75个女性样本，检测尿液FB1。尿FB1的几何平均值（95%可信区间）分别为35.0（18.8-65.2）、63.1（36.8-108.2）和147.4 (87.6-248.0)pg/mL，在低、中、高组中，高于检出限（设定为20 pg FB1/mL尿液）的样本频率分别为45%、80%和96%。高摄入量妇女的平均FB1水平是低摄入量组的3倍（F = 7.3; P = 0.0015）。尿FB1与玉米摄取量相关（p趋势= 0.001）；在调整了年龄、教育程度和居住地后，相关性仍然显著。这项研究表明，尿液FB1的测量对人类伏马菌素暴露评估足够敏感，可能是研究暴露相关健康影响的有价值的工具。
Fumonisins are mycotoxins produced by Fusarium spp. and commonly contaminate maize and maize products worldwide. Fumonisins are rodent carcinogens and have been associated with human esophageal cancer. However, the lack of a valid exposure biomarker has hindered both the assessment of human exposure and the evaluation of disease risk. A sensitive liquid chromatography-mass spectrometry method to measure urinary fumonisin B1 (FB1) following extraction on Oasis MAX cartridges was established and applied to urine samples from women in a cohort recruited in Morelos County, Mexico. Urinary FB1 was compared with dietary information on tortilla consumption. FB1 recovery in spiked samples averaged 94% as judged by deuterium-labeled FB1 internal standard. Urinary FB1 was determined in 75 samples from women selected based on low, medium, or high consumption of maize-based tortillas. The geometric mean (95% confidence interval) of urinary FB1 was 35.0 (18.8-65.2), 63.1 (36.8-108.2), and 147.4 (87.6-248.0) pg/mL and the frequency of samples above the detection limit (set at 20 pg FB1/mL urine) was 45%, 80%, and 96% for the low, medium, and high groups, respectively. Women with high intake had a 3-fold higher average FB1 levels compared with the "low intake" group (F = 7.3; P = 0.0015). Urinary FB1 was correlated with maize intake (P-trend = 0.001); the correlation remained significant after adjusting for age, education, and place of residence. This study suggests that measurement of urinary FB1 is sufficiently sensitive for fumonisin exposure assessment in human populations and could be a valuable tool in investigating the associated health effects of exposure.