Separation of ephedrine stereoisomers by molecularly imprinted polymers--influence of synthetic conditions and mobile phase compositions on the chromatographic performance.
Separation of ephedrine stereoisomers by molecularly imprinted polymers--influence of synthetic conditions and mobile phase compositions on the chromatographic performance.
复制标题
DOI：
10.1039/b202295h
复制
发表时间：
2002-10
期刊：
影响因子：
--
通讯作者：
X. Dong;Hui Sun;Xianyu Lu;Haibo Wang;Shuxia Liua;Ning Wang
中科院分区：
文献类型：
--
作者：
X. Dong;Hui Sun;Xianyu Lu;Haibo Wang;Shuxia Liua;Ning Wang
采用分子印迹聚合物分离麻黄碱立体异构体，考察了影响分离选择性和柱效的因素。以季戊四醇三丙烯酸酯为交联剂，氯仿为致孔剂合成的聚合物具有最好的整体分离性能。核磁共振和层析分析表明，聚合物固定相与麻黄素的主要结合作用是离子和氢键相互作用。对流动相组成对高效液相色谱分析影响的研究表明，甲醇-水缓冲液是适合分离的流动相，可通过调节pH、离子强度和水含量来优化色谱分析。
Separation of ephedrine stereoisomers by molecularly imprinted polymers was performed to study the factors that affect the selectivity and column efficiency. The polymer synthesized with pentaerythritol triacrylate as the cross-linker and chloroform as the porogen was found to have the best overall separation performance. Investigation of the recognition mechanism by NMR and chromatographic analysis revealed that the major binding forces between the polymer stationary phase and ephedrine are the ionic and hydrogen bonding interactions. Studies of the influence of mobile phase compositions on the HPLC analysis have shown that a methanol-aqueous buffer was the suitable mobile phase for the separation in which pH, ionic strength and water content can be adjusted to optimize the chromatographic analysis.