Impact of ion magnetron motion on electron capture dissociation Fourier transform ion cyclotron resonance mass spectrometry
Impact of ion magnetron motion on electron capture dissociation Fourier transform ion cyclotron resonance mass spectrometry
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DOI：
10.1016/j.ijms.2005.11.014
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发表时间：
2006-10-01
影响因子：
1.8
通讯作者：
Marshall, Alan G.
中科院分区：
文献类型：
--
作者：
Tsybin, Yury O.;Hendrickson, Christopher L.;Marshall, Alan G.
在9.4T傅里叶变换离子回旋共振（FT-ICR）质谱仪中，电子捕获解离（ECD）效率随离子和电子注入时间间隔呈周期性变化。所观察到的调制频率与离子磁控管频率的相关性在1%以内，这很可能是由于离子束和电子束之间的未对准。最佳的ECD条件是通过正确地调整电子注入与离子磁控管的运动。由ICR阱径向电场的变化引起的捕获离子云的位移降低了ECD效率调制幅度。实验直接表明，只有在电子注入时与电子相互作用的离子才参与ECD反应。(C)2006 Elsevier B. V.保留所有权利。
Electron capture dissociation (ECD) efficiency in a 9.4 T Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer varies periodically with the time interval between ion and electron injection. The observed modulation frequency correlates to within 1% with ion magnetron frequency, most probably due to misalignment between the ion beam and the electron beam. The optimum ECD conditions are obtained by correctly phasing electron injection with the ion magnetron motion. Displacement of the trapped ion cloud by variation of the ICR trap radial electric field decreases ECD efficiency modulation amplitude. Experiments directly suggest that only ions interacting with electrons at the moment of electron injection participate in ECD reactions. (C) 2006 Elsevier B.V. All rights reserved.