Detection of antibodies to a recombinant gag protein derived from human endogenous retrovirus clone 4-1 in autoimmune diseases
Detection of antibodies to a recombinant gag protein derived from human endogenous retrovirus clone 4-1 in autoimmune diseases
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DOI：
10.1089/vim.1997.10.137
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发表时间：
1997-01-01
期刊：
影响因子：
2.2
通讯作者：
Maruyama, N
中科院分区：
文献类型：
--
作者：
Hishikawa, T;Ogasawara, H;Maruyama, N
为探讨人类内源性逆转录病毒(HERV)在自身免疫性疾病中的作用，我们利用杆状病毒载体系统，从HERV家族的4-1克隆中制备了重组P30(Gag)蛋白。该P30(Gag)蛋白(CA41B)如预期的那样约为30 kDa，并与从小鼠和猫白血病病毒中纯化的P30(GAG)抗体反应。这一结果表明，P30(GAG)的抗原决定簇在CA41B中是保守的。用免疫印迹法检测自身免疫性疾病患者血清中P30抗体(GAG)。CA41B在48.3%的系统性红斑狼疮(SLE)、35.0%的干燥综合征(SS)和33.3%的混合性结缔组织病(MCTD)患者中检测到抗P30(GAG)抗体，而在健康人中未检测到抗P30(GAG)抗体。提示HERVP30(GAG)或其他与CA41B具有相同抗原决定簇的逆转录病毒P30(GAG)蛋白可能在这些疾病中起作用。虽然在自身免疫性疾病中检测到HERV P30(GAG)抗体是参与HERV蛋白参与的间接证据，但本研究表明，自身免疫性疾病患者使用重组HERV蛋白而不是基于HERV或其他物种逆转录病毒蛋白的合成肽来产生HERV P30(GAG)抗体。
To investigate whether human endogenous retroviruses (HERV) contribute to autoimmune diseases, we prepared a recombinant p30(gag) protein derived from clone 4-1 of the HERV family, using a baculovirus-vector system. This p30(gag) protein (CA41B) was approximately 30 kDa, as expected, and reacted with antibodies for p30(gag) purified from both murine and feline leukemia virus. This result suggested that the antigenic determinant for p30(gag) was well conserved in CA41B. Analysis of serum antibodies to p30(gag) in patients with autoimmune diseases was done by Western blotting. CA41B detected anti-pg30(gag) antibodies in 48.3% of systemic lupus erythematosus (SLE) patients, 35.0% of Sjogren's syndrome (SS) patients, and 33.3% of mixed connective tissue disease (MCTD) patients, whereas no anti-p30(gag) antibodies were found in healthy subjects. This suggested that HERV p30(gag) or other retroviral p30(gag) proteins possessing the same antigenic determinant as CA41B may play a role in these diseases. Although detection of antibodies to HERV p30(gag) in autoimmune diseases is indirect evidence that HERV proteins are involved, this study showed that patients with autoimmune diseases have antibodies to HERV p30(gag) using a recombinant HERV protein rather than synthetic peptides based on HERV or retroviral proteins of other species.