Nuclear size as estrogen-responsive chromatin quality parameter of mouse spermatozoa
Nuclear size as estrogen-responsive chromatin quality parameter of mouse spermatozoa
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DOI：
10.1016/j.ygcen.2013.07.018
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发表时间：
2013-11-01
影响因子：
2.7
通讯作者：
Cobellis, Gilda
中科院分区：
文献类型：
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作者：
Cacciola, Giovanna;Chioccarelli, Teresa;Cobellis, Gilda
最近，我们研究了内源性大麻素参与雄性精子细胞中发生的染色质重塑事件。事实上，我们已经证明，1 型大麻素受体 (Cnr1) 的基因失活会减少组蛋白位移和精子染色质质量指数（染色质浓缩和 DNA 完整性），从而对染色质重塑机制产生负面影响。相反，Cnr1 敲除 (Cnr1(-/-)) 雄性小鼠在接受雌激素治疗后，取代了组蛋白并恢复了染色质凝聚以及 DNA 完整性。在本研究中，通过利用 Cnr1(+/+)、Cnr(+/-) 和 Cnr1(-/-) 附睾精子样本，我们发现组蛋白保留与决定精子核大小伸长的精子染色质质量指数的低值直接相关。此外，我们证明雌激素通过促进组蛋白置换和染色质浓缩救援，能够有效地减少在 Cnr1(-/-) 精子中观察到的更大的核长度。根据我们的结果，我们建议核长度可用作形态学参数，有助于筛选出染色质质量低的精子。 (C) 2013 Elsevier Inc. 保留所有权利。
Recently, we have investigated the endocannabinoid involvement in chromatin remodeling events occurring in male spermatids. Indeed, we have demonstrated that genetic inactivation of the cannabinoid receptor type 1 (Cnr1) negatively influences chromatin remodeling mechanisms, by reducing histone displacement and indices of sperm chromatin quality (chromatin condensation and DNA integrity). Conversely, Cnr1 knock-out (Cnr1(-/-)) male mice, treated with estrogens, replaced histones and rescued chromatin condensation as well as DNA integrity. In the present study, by exploiting Cnr1(+/+), Cnr(+/-) and Cnr1(-/-) epididymal sperm samples, we show that histone retention directly correlates with low values of sperm chromatin quality indices determining sperm nuclear size elongation. Moreover, we demonstrate that estrogens, by promoting histone displacement and chromatin condensation rescue, are able to efficiently reduce the greater nuclear length observed in Cnr1(-/-) sperm. As a consequence of our results, we suggest that nucleus length may be used as a morphological parameter useful to screen out spermatozoa with low chromatin quality. (C) 2013 Elsevier Inc. All rights reserved.