VIRUSES ISOLATED FROM BATS IN JAPAN
VIRUSES ISOLATED FROM BATS IN JAPAN
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DOI：
10.1007/bf01315626
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发表时间：
1977-01-01
影响因子：
2.7
通讯作者：
KITAOKA, M
中科院分区：
文献类型：
--
作者：
MIURA, T;KITAOKA, M
从蝙蝠的血液、脾脏、肝脏和肾脏中分离病毒。从日本不同地区收集的838只蝙蝠中分离出9种病毒（1.1%）。其中两个分离物（TOK-1350和TOK-1362）是从福岛县的Vespertilio supernas中分离的。他们在脑内接种后3-5天内杀死乳鼠。剩余的7个分离物都可通过Millipore过滤器（0.22 μ）过滤。对乳鼠有致病性，但对成年鼠无致病性。其中KY-5株耐乙醚，TOK-292株部分耐乙醚，均对脱氧胆酸钠（SDC）耐药，KY-663株对SDC部分耐药。蔗糖丙酮（SA）抗原的每一个新的分离株没有显示与鹅红细胞的血凝（HA），即使鱼精蛋白或超声波处理后。7株菌中有4株具有补体结合活性。TOK-949和VS-208在同一地点采集，可能具有相同的CF活性。其余5个菌株明显不同。YF-17 D和Rio Bravo与抗KY-5、TOK-949、TOK-1280、VS-820和VeJ-822的抗血清均产生高HI [血凝抑制]滴度。它们似乎是黄病毒，虽然它们不同于日本脑炎病毒（JEF）株。1-10%的蝙蝠血清含有抗JEV的抗体。
Virus isolation was attempted from the blood, spleen, liver and kidneys of bats. Nine viruses (1.1%) were isolated so far from 838 bats collected in different places in Japan. Two of the isolates (TOK-1350 and TOK-1362) were isolated from Vespertilio supernas collected in Fukushima Prefecture. They killed suckling mice within 3-5 days after intracerebral inoculation. The remaining 7 isolates were all filtrable through Millipore filters (0.22 .mu.) and were pathogenic for suckling mice but not adults. One of 7 strains (KY-5) was ether resistant and another (TOK-292) was partially ether resistant and both were resistant to sodium deoxycholate (SDC); another (KY-663) was partially resistant to SDC. The sucrose-acetone (SA) antigen of each new isolate did not show hemagglutination (HA) with goose red cells, even after protamine or ultrasonic treatment. Complement fixing (CF) activity was found in 4 of the 7 strains. Both TOK-949 and VS-208 were collected at the same place and probably have the same CF activity. The remaining 5 strains are apparently different. Both YF-17D and Rio Bravo gave high HI [hemagglutination-inhibition] titer with antisera against KY-5, TOK-949, TOK-1280, VS-820 and VeJ-822. They seemed to be flaviviruses although they were distinct from Japanese encephalitis virus (JEF) strains. From 1-10% of bat sera contained antibody against JEV.