Evaluation of introgressive hybridization among Cervidae in Japan’s Kinki District via two novel genetic markers developed from public NGS data
Evaluation of introgressive hybridization among Cervidae in Japan’s Kinki District via two novel genetic markers developed from public NGS data
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通过根据公共 NGS 数据开发的两种新型遗传标记评估日本近畿地区的鹿科动物渐渗杂交
DOI：
10.1002/ece3.5131
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发表时间：
2019
影响因子：
2.6
通讯作者：
Tamate HB
中科院分区：
文献类型：
--
作者：
Matsumoto Y;Takagi T;Koda R;Tanave A;Yamashiro A;Tamate HB
本地种群与引进物种的杂交和回交会导致基因渗入和遗传改变。在这项研究中，我们评估了43只鹿的渐渗情况，这些鹿来自日本近基区冲之岛附近的一个潜在杂交带。这个地区见证了一个杂交种群的迁徙（台湾梅花鹿[Cervus nippon taiouanus]和其他鹿种的杂交），它可能与本土的日本梅花鹿（c.n. centralis）交配。我们使用现有的线粒体细胞色素bgene遗传标记和两个新的核DNA标记，这些标记是利用公开的下一代测序数据开发的。我们发现了一只大陆鹿的线粒体单倍型与台湾梅花鹿相同，核杂合序列与台湾梅花鹿和日本梅花鹿相同。这表明大陆鹿是冲之岛种群和本地鹿的杂交后代。而在其他42个样本中只发现了日本梅花鹿的序列，表明基因渗入程度有限。然而，在冲之岛种群中引入前后的杂交可能导致日本梅花鹿的多物种渗入，对遗传完整性产生负面影响。我们开发了一种基于聚合酶链反应-限制性片段长度多态性的简单测试来检测自然群体中的基因渗入。该方法可加快近畿地区梅花鹿的遗传监测。综上所述，为了防止日本梅花鹿的进一步入侵和保持遗传完整性，我们建议在冲之岛周围建立围栏以限制迁徙，并对本地鹿进行持续的遗传监测。
Hybridization and backcrossing of native populations with introduced species can lead to introgression and genetic alteration. In this study, we evaluated introgression in 43 deer from a potential hybrid zone around Okinoshima Island, Kinki District, Japan. This region witnessed the migration of a hybrid population (cross between the Formosan sika deer [Cervus nippon taiouanus] and other deer species) that could potentially breed with the native Japanese sika deer (C. n. centralis). We used an existing genetic marker for the mitochondrialcytochrome bgene and two novel markers for nuclear DNA, developed using publicly available next‐generation sequencing data. We identified one mainland deer with a mitochondrial haplotype identical to that of the Formosan sika deer as well as nuclear heterozygous sequences identical to those of Formosan and Japanese sika deer. This suggests that the mainland deer is a hybrid offspring of the Okinoshima population and native deer. However, only Japanese sika deer sequences were found in the other 42 samples, indicating limited introgression. Nevertheless, hybridization pre‐ and postintroduction in the Okinoshima population could cause multispecies introgression among Japanese sika deer, negatively affecting genetic integrity. We developed a simple test based on polymerase chain reaction–restriction fragment length polymorphism to detect introgression in natural populations. Our method can accelerate genetic monitoring of Japanese sika deer in Kinki District. In conclusion, to prevent further introgression and maintain genetic integrity of Japanese sika deer, we recommend establishing fences around Okinoshima Island to limit migration, besides a continued genetic monitoring of the native deer.