Structure of a Yeast 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex
Structure of a Yeast 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex
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DOI：
10.1038/nsmb.2963
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发表时间：
2015-03-01
影响因子：
16.8
通讯作者：
Ban, Nenad
中科院分区：
文献类型：
--
作者：
Aylett, Christopher H. S.;Boehringer, Daniel;Ban, Nenad
真核生物翻译起始需要在40S核糖体亚基上协同组装一个大的蛋白质复合物。我们已经解决了一个芽殖酵母起始复合物的冷冻EM，允许放置以前的结构eIF1，eIF1A，eIF3a，eIF3b和eIF3c。我们的结构突出了与哺乳动物eIF3相比，起始复合物与核糖体结合的差异，证明了eIF3j和eIF1A之间的直接接触，并揭示了eIF3亚基之间的相互作用网络。
Eukaryotic translation initiation requires cooperative assembly of a large protein complex at the 40S ribosomal subunit. We have resolved a budding yeast initiation complex by cryo-EM, allowing placement of prior structures of eIF1, eIF1A, eIF3a, eIF3b and eIF3c. Our structure highlights differences in initiation-complex binding to the ribosome compared to that of mammalian eIF3, demonstrates a direct contact between eIF3j and eIF1A and reveals the network of interactions between eIF3 subunits.