Developmental expression of paraoxonase 2
Developmental expression of paraoxonase 2
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DOI：
10.1016/j.cbi.2016.04.001
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发表时间：
2016-11-25
影响因子：
5.1
通讯作者：
Costa, Lucio G.
中科院分区：
文献类型：
--
作者：
Garrick, Jacqueline M.;Dao, Khoi;Costa, Lucio G.
对氧磷酶2(PON2)是对氧磷酶基因家族的成员，也包括PON1和PON3。PON2是一种乳糖酶，具有抗菌和抗氧化的特性。在细胞水平上，PON2定位于线粒体和内质网膜，在那里它清除活性氧物种。PON2特别令人感兴趣，因为它是唯一在脑组织中表达的对氧磷酶，似乎在减轻大脑中的氧化应激方面发挥着关键作用。本研究的目的是研究PON2在发育过程中在小鼠脑和肝脏中的蛋白和mRNA水平的表达，以确定对氧化应激敏感的潜在年龄窗口，并比较肝脏PON2和PON1和PON3的表达。总体而言，PON2在新生小鼠脑中的表达较低，并随着年龄的增长而增加，直到出生后21天(PND)，在出生后30天和60天观察到显著下降。相反，肝脏中PON2的水平随着年龄的增长而持续增加，与PON1和PON3相似。在非人类灵长类动物的大脑样本中也研究了PON2蛋白水平，PON2蛋白水平随着年龄的增长而增加，直到婴儿阶段，在青少年阶段下降，这反映了在小鼠大脑中观察到的结果。PON2的这些可变表达水平表明，由于大脑中PON2的水平较低，新生和年轻成年动物可能更容易受到氧化剂的神经侮辱。(C)2016爱思唯尔爱尔兰有限公司。保留所有权利。
Paraoxonase 2 (PON2) is a member of the paraoxonase gene family also comprising PON1 and PON3. PON2 functions as a lactonase and exhibits anti-bacterial as well as antioxidant properties. At the cellular level, PON2 localizes to the mitochondrial and endoplasmic reticulum membranes where it scavenges reactive oxygen species. PON2 is of particular interest as it is the only paraoxonase expressed in brain tissue and appears to play a critical role in mitigating oxidative stress in the brain. The aim of this study was to investigate the expression of PON2 at the protein and mRNA level in the brain and liver of mice through development to identify potential age windows of susceptibility to oxidative stress, as well as to compare expression of hepatic PON2 to expression of PON1 and PON3. Overall, PON2 expression in the brain was lower in neonatal mice and increased with age up to postnatal day (PND) 21, with a significant decrease observed at PND 30 and 60. In contrast, the liver showed continuously increasing levels of PON2 with age, similar to the patterns of PON1 and PON3. PON2 protein levels were also investigated in brain samples from non-human primates, with PON2 increasing with age up to the infant stage and decreasing at the juvenile stage, mirroring the results observed in the mouse brain. These variable expression levels of PON2 suggest that neonatal and young adult animals may be more susceptible to neurological insult by oxidants due to lower levels of PON2 in the brain. (C) 2016 Elsevier Ireland Ltd. All rights reserved.