Involvement of vanilloid receptor VR1 and prostanoids in the acid-induced writhing responses of mice
Involvement of vanilloid receptor VR1 and prostanoids in the acid-induced writhing responses of mice
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DOI：
10.1016/s0024-3205(01)01374-1
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发表时间：
2001-11-02
期刊：
影响因子：
6.1
通讯作者：
Oh-ishi, S
中科院分区：
文献类型：
--
作者：
Ikeda, Y;Ueno, A;Oh-ishi, S
我们发现腹腔注射有机酸，如丙酸和乳酸，能够在小鼠中产生与乙酸类似的扭体反应。这些酸诱导的扭体反应显着衰减辣椒平，VR 1受体特异性拮抗剂，但苯基苯醌诱导的一个没有，这表明酸，而不是苯基苯醌激活VRI受体，这是参与多模态疼痛感知。缓激肽B-2受体拮抗剂Hoe 140也能抑制酸诱导的扭体反应。此外，这些扭体反应显着抑制新生儿治疗后，辣椒素，这种治疗是已知的破坏外周感觉传入C-纤维。辣椒平和Hoe 140没有进一步减弱辣椒素处理的小鼠已经减少的扭体反应，表明酸刺激VRI和缓激肽B2受体在包括感觉传入C-纤维的通路中。另一方面，吲哚美辛进一步显着抑制辣椒素处理的动物的扭体数，这表明酸诱导的疼痛感知不仅需要前列腺素受体通过辣椒素敏感的C-纤维的途径，但也在其他感觉通路。这些结果提供了第一个证据，通过感觉C-纤维的酸诱导的炎症性疼痛知觉的香草酸受体参与除了已知的介质缓激肽，神经激肽，前列腺素。(C)2001 Elsevier Science Inc. All rights reserved.
We found that intraperitoneal injection of organic acids, such as propionic and lactic acid, are able to develop writhing responses in mice similarly as that of acetic acid. These acid-induced writhing reactions were significantly attenuated by capsazepine, a VR1 receptor-specific antagonist, but the phenylbenzoquinone-induced one was not, suggesting that the acids but not phenylbenzoquinone activate the VRI receptor, which is involved in polymodal pain perception. Hoe 140, a bradykinin B-2 receptor antagonist, also suppressed the acid-induced writhing response. Furthermore, these writhing responses were significantly suppressed after neonatal treatment with capsaicin, which treatment is known to destroy peripheral sensory afferent C-fibers. Capsazepine and Hoe 140 did not further attenuate the already reduced writhing responses of capsaicin-treated mice, suggesting that the acids stimulate the VRI and the bradykinin B2 receptor in the pathway comprising sensory afferent C-fibers. On the other hand, indomethacin further significantly suppressed the writhing number of the capsaicin-treated animals, suggesting that the acid-induced pain perception requires prostanoid receptors not only in the pathway via capsaicin-sensitive C-fibers but also in other sensory pathways. These results provide the first evidence for the involvement of the vanilloid receptor in the acid-induced inflammatory pain perception via sensory C-fibers in addition to the known mediators bradykinin, neurokinins, and prostanoids. (C) 2001 Elsevier Science Inc. All rights reserved.