Common replication strategies emerging from the study of diverse groups of positive-strand RNA viruses.
Common replication strategies emerging from the study of diverse groups of positive-strand RNA viruses.
复制标题
对不同组的正链 RNA 病毒的研究中出现的常见复制策略。
DOI：
10.1007/978-3-7091-9326-6_18
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
T. Hall
中科院分区：
文献类型：
--
作者：
G. Pogue;C. Huntley;T. Hall
利用雀科花叶病毒（BMV）、辛德比斯病毒和脊髓灰质炎病毒进行的研究提供了证据，表明不同的植物和动物正链RNA病毒群具有非常相似的复制策略。病毒编码的非结构蛋白中几个功能域的保守性表明，尽管这些和相互作用的宿主成分的确切特征因每种病毒而异，但它们采用相似的RNA复制机制。对于（+）链复制，已鉴定出基因组（+）链5'末端的顺式作用序列基序和RNA二级结构的相似性，并已证明它们参与宿主因子的结合。提出的BMV RNA复制模型表明，这些因子与双链复制中间体中的内部控制区（ICR）序列基序的结合释放了（-）链上的单链3'端，这可能是基因组（+）链合成起始所必需的。BMV基因组内部的ICR序列也被发现是有效复制所必需的。所有（+）链病毒的特征是过量基因组（+）过（-）链RNA的不对称产生，这可能是通过招募额外的宿主因子将复制酶从（-）链合成能力过渡到（+）链合成能力来完成的。
Studies using brome mosaic virus (BMV), Sindbis virus and poliovirus have provided evidence that disparate groups of plant and animal positive strand RNA viruses have remarkably similar replication strategies. The conservation of several functional domains within virus-encoded nonstructural proteins implies that, although the precise character of these and interacting host components varies for each virus, they employ similar mechanisms for RNA replication. For (+) strand replication, similarities in cis-acting sequence motifs and RNA secondary structures within 5' termini of genomic (+) strands have been identified and have been shown to participate in binding of host factors. The model presented for replication of BMV RNA suggests that binding of these factors to internal control region (ICR) sequence motifs in the double-stranded replication intermediate releases a single-stranded 3' terminus on the (-) strand that may be essential for initiation of genomic (+) strand synthesis. ICR sequences internal to the BMV genome were also found to be required for efficient replication. Asymmetric production of excess genomic (+) over (-) strand RNA, characteristic of all (+) strand viruses, may be accomplished through transition of the replicase from competence for (-) to (+) strand synthesis by the recruitment of additional host factors.
雀麦花叶病毒 RNA 的 ICR2 基序中的点突变会削弱 ( ) 链复制。
影响因子：
3.7
作者：
Pogue,GP;Marsh,LE;Hall,TC
通讯作者：
Hall,TC
使用生物素化 RNA 探针分析病毒感染中的 RNA 稳定性和 (-) 链含量。
影响因子：
3.1
作者：
Rao,AL;Huntley,CC;Marsh,LE;Hall,TC
通讯作者：
Hall,TC