Research Update: A minimal region of squid reflectin for vapor-induced light scattering
Research Update: A minimal region of squid reflectin for vapor-induced light scattering
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DOI：
10.1063/1.4997199
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发表时间：
2017-12-01
期刊：
影响因子：
6.1
通讯作者：
Crookes-Goodson, Wendy J.
中科院分区：
文献类型：
--
作者：
Dennis, Patrick B.;Singh, Kristi M.;Crookes-Goodson, Wendy J.
反射蛋白是在头足类动物的光操纵细胞中发现的一个蛋白质家族。这些蛋白质由一系列保守的重复序列组成，这些重复序列含有被认为对功能很重要的高度代表性的氨基酸。先前的研究表明，投射到薄膜中的重组反射体产生的结构颜色可以通过变化的环境条件动态调节。在这项研究中，我们展示了暴露于一系列水蒸气脉冲后反射膜的光散射。对薄膜表面形貌的分析表明，光散射的诱导伴随着反射体自组装成微纳米尺度的特征。使用简化策略，我们确定哪些反射重复和亚重复是必要的这些事件后，水蒸气脉冲。通过这种方法，我们在反射物中确定了一个单独代表的23个氨基酸区域，足以概括在全长蛋白质薄膜中观察到的光散射特性。最后，利用反射膜的水稳定性表明，预暴露于不同pH的缓冲液可以调节水蒸气脉冲诱导光散射和蛋白质自组装的能力。(c) 2017作者。
Reflectins are a family of proteins found in the light manipulating cells of cephalopods. These proteins are made up of a series of conserved repeats that contain highly represented amino acids thought to be important for function. Previous studies demonstrated that recombinant reflectins cast into thin films produced structural colors that could be dynamically modulated via changing environmental conditions. In this study, we demonstrate light scattering from reflectin films following exposure to a series of water vapor pulses. Analysis of film surface topography shows that the induction of light scatter is accompanied by self-assembly of reflectins into micro-and nanoscale features. Using a reductionist strategy, we determine which reflectin repeats and sub-repeats are necessary for these events following water vapor pulsing. With this approach, we identify a singly represented, 23-amino acid region in reflectins as being sufficient to recapitulate the light scattering properties observed in thin films of the full-length protein. Finally, the aqueous stability of reflectin films is leveraged to show that pre-exposure to buffers of varying pH can modulate the ability of water vapor pulses to induce light scatter and protein self-assembly. (c) 2017 Author(s).