Differential endothelial migration and proliferation to basic fibroblast growth factor and vascular endothelial growth factor.

Differential endothelial migration and proliferation to basic fibroblast growth factor and vascular endothelial growth factor.
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DOI：
10.3109/08977199609034566
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发表时间：
1996
期刊：
Growth factors
影响因子：
1.8
通讯作者：
A. Yoshida;B. Anand-Apte;B. Zetter
A. Yoshida;B. Anand-Apte;B. Zetter
中科院分区：
生物学4区
文献类型：
--
作者：
A. Yoshida;B. Anand-Apte;B. Zetter

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新生血管是多种病理过程的特征。比较血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(BFGF)对人脐静脉内皮细胞(HUVEC)迁移和增殖的影响。血管内皮细胞生长因子和碱性成纤维细胞生长因子在相似浓度(1/2max.血管内皮生长因子约为1.0 ng/ml，碱性成纤维细胞生长因子约为5.0 ng/ml)。然而，当浓度为1和10 ng/ml时，血管内皮生长因子刺激的迁移能力是碱性成纤维细胞生长因子的两倍(p&lt；0.05)。相比之下，碱性成纤维细胞生长因子诱导增殖的效率是血管内皮生长因子的四倍(1/2最大。1 ng/ml和1.4 ng/ml)。棋盘式迁移分析显示bFGF主要为趋化运动模式，而VEGF主要为趋化作用。在单层增殖和迁移实验中，血管内皮生长因子和碱性成纤维细胞生长因子没有协同作用。三种血管生成抑制剂，α-干扰素、TNP-470和血小板因子-4可抑制血管内皮生长因子和碱性成纤维细胞生长因子诱导的细胞迁移。这些结果表明，血管内皮生长因子和碱性成纤维细胞生长因子是通过不同机制刺激内皮细胞迁移的化学诱导剂，两者都可以被已知的血管生成抑制剂所抑制。
Neovascularization is a feature of a variety of pathological processes. We compared the characteristics of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) on migration and proliferation of human umbilical vein endothelium (HUVEC). Both VEGF and bFGF induced endothelial cell migration at similar concentrations (1/2 max. VEGF = approximately 1.0 ng/ml, bFGF = approximately 5.0 ng/ml). However, VEGF-stimulated migration was two-fold greater than bFGF at 1 and 10 ng/ml (p < 0.05). In contrast, bFGF induced proliferation four-fold more effectively than VEGF (1/2 max. 1 ng/ml and 1.4 ng/ml respectively). Checkerboard migration assays for bFGF showed a predominantly chemokinetic pattern, whereas VEGF was predominantly chemotactic. VEGF and bFGF were not synergistic in monolayer proliferation and migration assays. Three angiogenesis inhibitors, alpha-interferon, TNP-470, and platelet factor-4, inhibited VEGF and bFGF induced cell migration. These results indicate that VEGF and bFGF are chemoattractants that stimulate endothelial migration by different mechanisms and that both can be inhibited by known angiogenesis inhibitors.