Nucleotide sequence of the putative human tyrosinase pseudogene.
Nucleotide sequence of the putative human tyrosinase pseudogene.
复制标题
假定的人酪氨酸酶假基因的核苷酸序列。
DOI：
10.1620/tjem.163.295
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
S. Shibahara
中科院分区：
文献类型：
--
作者：
A. Takeda;J. Matsunaga;Y. Tomita;H. Tagami;S. Shibahara
我们克隆并测序了人酪氨酸酶假基因，该基因与人酪氨酸酶基因的外显子4和外显子5及其侧翼内含子有98%以上的核苷酸同源性。由于具有如此高的同源性，因此可以通过聚合酶链式反应从基因组DNA中扩增出酪氨酸酶基因及其假基因。因此，提出的核苷酸序列使我们能够区分酪氨酸酶基因和它的相关序列，对于眼皮肤白化病的基因诊断是非常有价值的。
We have cloned and sequenced the putative human tyrosinase pseudogene, which shares more than 98% nucleotide homology with exon 4 and exon 5 of the human tyrosinase gene including their flanking introns. Because of such a high homology, both the tyrosinase gene and its pseudogene could be amplified from genomic DNA by polymerase chain reaction. The nucleotide sequences presented thus enable us to discriminate the tyrosinase gene from its related sequences and are invaluable for a gene diagnosis of oculocutaneous albinism.